反相高效液相色谱法测定鲨肝醇片的含量  

Determination of Batilol Tablets by RP-HPLC

作　　者：宋玉琴 宋青 董芳 SONG Yu-qin;SONG Qing;DONG Fang(Zibo Institute for Food and Drug Control,Zibo 255086,China)

机构地区：[1]淄博市食品药品检验研究院,山东淄博255086

出　　处：《食品与药品》2022年第2期158-160,共3页Food and Drug

摘　　要：目的 建立一种测定鲨肝醇片含量的方法。方法 采用高效液相色谱(HPLC)法,色谱柱为C;柱,流动相为乙腈-水(90:10),流速为1.0 ml/min,检测波长为200 nm,柱温为30℃。结果 鲨肝醇检测质量浓度线性范围为0.26~5.2 mg/ml(r=0.9999),检测限和定量限分别为775,2.58μg/ml,平均回收率为98.73%(RSD=0.84%,n=6)。结论 该方法准确、灵敏、简便,可用于鲨肝醇片的质量控制。Objective To establish a method for determination of Batilol Tablets. Methods HPLC was adopted.The determination was performed on a C;column with mobile phase consisted of acetonitrile-water(90:10) at the flow rate of 1.0 ml/min. The detection wave-length was set at 200 nm. The column temperature was 30 ℃. Results The linear range of batilol were 0.26-5.2 mg/ml(r=0.9999). The limit of detection was 775 ng/ml and the limit of quantification was 2.58 μg/ml. The average recovery was 98.73 %(RSD=0.84 %, n=6). Conclusion The method is accurate, sensitive and simple, which can be used for the quality control of Batilol Tablets.

关 键 词：鲨肝醇 高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]