大鼠腹股沟区血管显微吻合训练的可行性研究  

Feasibility study on the training method of microvascular anastomosis in inguinal region of rats

作　　者：李唐[1,2] 孙杨[1] 杨建华 张文超 佟小光[1] Li Tang;Sun Yang;Yang Jianhua;Zhang Wenchao;Tong Xiaoguang(Clinical College of Neurology,Neurosurgery and Neurorehabilitation,Tianjin Medical University,Department of Neurosurgery,Tianjin Huanhu Hospital,Tianjin 300350,China;Department of Neurosurgery,the First Affiliated Hospital of Guangxi Medical University)

机构地区：[1]天津医科大学神经内外科及神经康复临床学院,天津市环湖医院神经外科,天津300350 [2]广西医科大学第一附属医院神经外科

出　　处：《中华神经外科杂志》2022年第3期305-309,共5页Chinese Journal of Neurosurgery

基　　金：天津市科委重大专项(18ZXDBSY00180)。

摘　　要：目的探讨应用大鼠腹股沟区血管进行显微血管吻合训练的可行性及其应用价值。方法随机选取35只雄性SD大鼠,进行腹股沟区显微血管吻合术,共70侧。根据吻合方式不同分组,A组为端端吻合组,包括A1组(股动脉端端吻合组,采用两点定位法)、A2组(股动脉端端吻合组,采用单向缝合法);B组为半环式旁路移植端侧吻合组;C组为动静脉分流端侧吻合组,包括C1组(股动脉-股静脉端侧吻合组)、C2组(股静脉-股动脉端侧吻合组);D组为动静脉侧侧吻合组。观察大鼠的一般状态和死亡率情况,并统计各组的吻合时间,计算总体即刻吻合口通畅率。结果共完成腹股沟区血管吻合术70侧,其中吻合口不通5侧,吻合口渗血4侧,即刻吻合口通畅率为93%(65侧)。35只大鼠术中、术后均无死亡,术后术侧肢体血运、活动均正常。各组吻合时间:A1、A2组分别为(36.5±7.6)min和(43.1±8.5)min,两组的差异有统计学意义(t=-2.09,P=0.048);B组为(93.9±6.7)min;C1、C2组分别为(45.1±8.6)min和(50.4±10.1)min,两组的差异无统计学意义(t=-1.92,P=0.178);D组为(34.5±4.0)min。结论采用大鼠腹股沟区血管可进行不同方式吻合术的训练,术中局部血管的分离简单、快速、安全,术后大鼠致残、致死率低,可作为显微血管吻合训练的理想模型。Objective To explore the feasibility and application value of microvascular anastomosis training with inguinal blood vessels in rats.Methods Seventy sides of 35 male Sprague-Dawley rats were used for microvascular anastomosis in the inguinal region.According to different anastomosis methods,70 sides were divided into 4 groups:group A(femoral artery end-to-end anastomosis group)including group A1(two-point anchoring method)and group A2(one-way up method);group B(half-ring group);group C(arteriovenous shunt end-to-side anastomosis group)including group C1(femoral artery to femoral vein group)and group C2(femoral vein to femoral artery group);group D(femoral artery to vein side-to-side anastomosis).General status and mortality were observed in 35 rats.The suturing time and immediate patency rate were documented.Results Seventy cases of inguinal microvascular anastomosis were successfully completed.There were 5 cases of anastomotic occlusion and 4 cases of anastomotic leakage.The immediate patency rate was 93%(65/70).None of the 35 rats died during or after the operation,and the blood supply and limb movement on the operation side were normal after operation.The anastomosis time of group A1 and group A2 was 36.5±7.6 min and 43.1±8.5 min respectively,and the difference between the two groups was statistically significant(t=-2.09,P=0.048).The anastomosis time of group B was 93.9±6.7 min.The anastomosis time of group C1 and group C2 group was 45.1±8.6 min and 50.4±10.1 min respectively,and the difference between the two groups was not statistically significant(t=-1.92,P=0.178).The anastomosis time of group D was 34.5±4.0 min.Conclusions Rat inguinal blood vessels can be used for training of anastomosis with various methods.The dissection of the inguinal blood vessels during operation is simple,fast and safe,and the postoperative disability and mortality rate of rats are low.It can be used as an ideal model for microvascular anastomosis training.

关 键 词：吻合术 外科 腹股沟 可行性研究 训练 大鼠 

分 类 号：R656.21[医药卫生—外科学]