山茶CjMYB1基因的克隆及表达分析  

Molecular Cloning and Expression Analysis of CjMYB1 in Camellia japonica

作　　者：黄虎 马先进 李思佳 李辛雷[1] 李纪元[1] 殷恒福[1] HUANG Hu;MA Xianjin;LI Sijia;LI Xinlei;LI Jiyuan;YIN Hengfu(Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China;2Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,杭州311400 [2]南京林业大学,南京210037

出　　处：《西北植物学报》2022年第3期381-389,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31870578)。

摘　　要：该研究通过序列比对分析,以野生红山茶和不同花色品种山茶为材料,采用PCR方法克隆CjMYB1基因,并通过生物信息学和表达分析对其进行初步研究,为深入研究山茶CjMYB1基因在花色形成和花发育过程的调控机理奠定理论基础。结果表明:(1)成功克隆获得山茶CjMYB1基因(GenBank登录号为OL347930),其开放阅读框长为879 bp,编码292个氨基酸,相对分子质量为33.17 kD;CjMYB1基因属于R2R3-MYB转录因子,且与拟南芥MYB基因家族的第7亚组处于同一分支。(2)荧光定量PCR分析发现,山茶CjMYB1基因在野生红山茶花芽中表达量最高,在萼片、花瓣、雄蕊和心皮中都有较高的表达量,推测其在山茶花器官发育中发挥着重要作用;在红色山茶品种中表达量较高,而在粉色、淡黄色、白色山茶品种中表达量较低,说明CjMYB1基因可能在红色山茶品种的花色苷合成途径中起到了关键作用。(3)亚细胞定位实验表明,CjMYB1蛋白定位在细胞核。In this study,based on sequence alignment analysis,we cloned the full-length CjMYB1 gene from wild Camellia japonica and performed gene expression analyses in Camellia varieties with different floral colors,which provided a fundamental basis for understanding the function of CjMYB1 underlying the floral color formation during flower development.The results showed that:(1)CjMYB1 gene(GenBank accession:OL347930)was successfully cloned.Its open reading frame length was 879 bp,encoding a 292 amino acids protein with relative molecular weight of 33.17 kD.CjMYB1 is found to be a R2R3-MYB transcription factor and is homologous to the subgroup 7 of Arabidopsis thaliana MYB gene family.(2)Quantitative real-time PCR analysis showed that CjMYB1 gene had the highest expression level in the floral buds of wild C.japonica,and had a relatively high expression level in sepals,petals,stamens and carpels,suggesting that CjMYB1 plays an important role of regulating the floral development.The expression level of CjMYB1 gene was higher in red Camellia varieties,but lower in pink,light yellow and white Camellia varieties,suggesting that CjMYB1 gene may play a key role in the anthocyanin synthesis pathway.(3)Subcellular localization experiments showed that CjMYB1 protein was located in the nucleus.

关 键 词：山茶 CjMYB1基因 基因克隆 表达分析 亚细胞定位 

分 类 号：Q785[生物学—分子生物学] Q786