^(60)Co γ射线全身均匀照射大鼠早期血浆辐射敏感脂质筛选研究  

Screening of radiation sensitive lipids in rat plasma at early stage after total body irradiation with ^(60)Co γ-rays

在线阅读下载全文

作  者:赵骅[1] 习聪 刘海翔 陆雪[1] 田梅[1] 刘青杰[1] Zhao Hua;Xi Cong;Liu Haixiang;Lu Xue;Tian Mei;Liu Qingjie(Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China)

机构地区:[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出  处:《中华放射医学与防护杂志》2022年第3期175-180,共6页Chinese Journal of Radiological Medicine and Protection

基  金:国家自然科学基金(82003393,82173463)。

摘  要:目的探索大鼠受到全身照射后血浆脂质代谢特征,为辐射生物标志物研究提供科学依据。方法非靶向脂质组学研究中,将50只SD大鼠分为6组,用0、1、2、3、5、8 Gy钴60γ射线进行全身照射;靶向脂质组学研究中,将25只大鼠分为5组,用0、0.5、2.5、4、6 Gy射线进行照射。照射后4 h,采集静脉血并分离血浆,筛选辐射敏感脂质并测定其浓度,进行受试者工作特征曲线(ROC)和剂量-效应关系分析。结果非靶向脂质组学研究中,共筛选出15个辐射差异脂质;经靶向脂质组学验证,其中7个可作为辐射敏感脂质。辐射敏感脂质ROC区分0 Gy组与>0 Gy组、<2 Gy组与≥2 Gy组样本曲线下面积(AUC)均>0.75;将其组合后进行ROC分析,AUC值提高为0.96和0.94。在0~6 Gy范围内,LysoPC(18:2)、LysoPC(22:0)、PC(18:0/18:2)、PE(18:2/16:0)和PE(18:2/18:0)浓度随照射剂量的上升而下降。结论大鼠受照后4 h,共筛选出7个血浆辐射敏感脂质,其组合可用于特定照射剂量分类,其中5个脂质具有良好的剂量-效应关系。Objective To explore the characteristics of lipid metabolism in rat plasma after total body irradiation(TBI)in order to provide scientific evidence of radiation biomarkers.Methods For the non-targeted lipidomics study,50 SD rats were divided into 6 groups and irradiated with 0,1,2,3,5 or 8 Gy ^(60)Co γ-rays,respectively.For the targeted lipidomics study,25 rats were divided into 5 groups and irradiated with 0,0.5,2.5,4 or 6 Gy.Venous blood samples were collected and plasma were separated 4 h after TBI.Radiation-sensitive lipids were screened and their concentrations were determined.Receiver operating characteristic curve(ROC)and dose-response were analyzed.Results A total of 15 radiation differential lipids were screened out based on non-targeted lipidomics study and 7 of them were identified as radiosensitive lipids by targeted lipidomics analysis.The ROC of radiosensitive lipids distinguished area under curve(AUC)of samples in 0 Gy group and>0 Gy group,<2 Gy group and≥2 Gy group were all>0.75.The AUC values were increased to 0.96 and 0.94 after the panel of radiation sensitive lipids ROC analysis.The concentrations of LysoPC(18:2),LysoPC(22:0),PC(18:0/18:2),PE(18:2/16:0)and PE(18:2/18:0)decreased with irradiation dose within 0-6 Gy.Conclusions A total of 7 plasma radiosensitive lipids in rat plasma were identified 4 h after TBI,and the panel of them could be used for specific dose classification.Five of the lipids had good dose-response relationship.

关 键 词:脂质组学 电离辐射 生物标志物 受试者工作特征曲线 剂量-效应 

分 类 号:R144.1[医药卫生—公共卫生与预防医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象