乌药matK基因的生物信息学及多样性分析  

Bioinformatics and diversity analysis of matK gene of Lindera aggregate

作　　者：袁莉霞 王芙蓉 刘姝 孙妍 楼天灵[1] YUAN Li-xia;WANG Fu-rong;LIU Shu;SUN Yan;LOU Tian-ling(Zhejiang Pharmaceutical College,Ningbo 315100,Zhejiang,China)

机构地区：[1]浙江医药高等专科学校,浙江宁波315100

出　　处：《湖北农业科学》2022年第6期157-160,共4页Hubei Agricultural Sciences

基　　金：浙江省基础公益研究计划项目(LGN21H280003);宁波市自然科学基金项目(2019A610414);浙江省大学生科技创新活动计划项目(2019R458001);浙江医药高等专科学校校级重点课题(119014)。

摘　　要：对乌药[Lindera aggregate(Sims)Kosterm]叶绿体matK基因编码的蛋白序列进行了分析,并以11种山胡椒属植物构建了系统进化树。结果表明,matK基因全长为1628 bp,含有14个开放阅读框;亲水指数为-0.200,为亲水蛋白;乌药与桠乌药(Lindera obtusiloba)和三股筋香(Lindera thomsonii)的亲缘性比较近,与山胡椒(Lindera glauca)亲缘性最远。研究结果可为乌药种质资源鉴别、分类及多样性研究提供参考。Taking chloroplast matK gene of Lindera aggregate(Sims)Kosterm as a research object.This paper analyzed the sequence characteristics of matK encoded protein and the phylogenetic trees of 11 species of Lindera were constructed.The results showed that the full length of matK gene was 1628 bp,and contained 14 open reading frames(ORFs);The hydrophilic index was-0.200,which belonged to hydrophilic protein;It was closely related to Lindera obtusiloba and Lindera thomsonii,and farthest to Lindera glauca.This study provides a basis for the identification,classification and diversity of Lindera aggregate.

关 键 词：乌药 MATK基因 种质鉴别 遗传多样性 

分 类 号：R931.5[医药卫生—生药学]