Extensive pollen immigration and no evidence of disrupted mating patterns or reproduction in a highly fragmented holm oak stand  

作　　者：Joaquín Ortego Raúl Bonal Alberto Muñoz JoséMiguel Aparicio 

机构地区：[1]Grupo de Investigación de la Biodiversidad Genética y Cultural,Instituto de Investigación en Recursos Cinegéticos-IREC(CSIC,UCLM,JCCM),Ronda de Toledo s/n,E-13005 Ciudad Real,Spain [2]Departamento de Ecología Evolutiva,Museo Nacional de Ciencias Naturales(CSIC),C/JoséGutiérrez Abascal 2,E-28006 Madrid,Spain [3]Departamento de Didáctica de las Ciencias Experimentales,Facultad de Educación,Universidad Complutense de Madrid,C/Rector Royo Villanova s/n,E-28040 Madrid,Spain [4]Centre de Recerca Ecològica i Aplicacions Forestals(CREAF),Cerdanyola del Vallès,E-08193 Barcelona,Spain

出　　处：《Journal of Plant Ecology》2014年第4期384-395,共12页植物生态学报（英文版）

基　　金：Postdoctoral JAE-Doc(Consejo Superior de Investigaciones Científicas)and Juan de la Cierva(Ministerio de Economía y Competitividad)contracts(to J.O.,R.B.and A.M.).Junta de Comunidades de Castilla-La Mancha and European Social Fund(PII1C09-0256-9052);CONSOLIDER-MICINN(CSD2008-00040);Ministerio de Ciencia e Innovación(CGL2008-00095/BOS).

摘　　要：Aims Forest fragmentation and reduced tree population densities can poten-tially have negative impacts on mating patterns,offspring genetic diversity and reproductive performance.the aim of the present study is to test these hypotheses comparing an extremely fragmented,low tree density(~0.02 trees/ha)holm oak(Quercus ilex l.)stand from Central spain with a nearby high tree density stand(~50 trees/ha).Methods We genotyped adult trees and seeds from the low-density stand(436 seeds from 15 families)and the high-density stand(404 seeds from 11 families)using nine microsatellite markers.With these data,we performed paternity analyses,determined pollen flow,mating patterns and pollen pool structure,and estimated progeny genetic diversity in both stands.We also studied seed set and production and performed a pollen supplementation experiment to determine whether reduced tree density has limited foreign pollen availability.Important Findings We have found extensive pollen immigration(>75%)into the low tree density stand and monte Carlo simulations revealed that pollen moves larger distances than expected from null models of random dispersal.mating patterns and differentiation of pollen pools were similar in the high-density stand and the low-density stand but we found higher inter-annual differentiation of pollen pools in the for-mer.Progeny genetic diversity and self-fertilization rates did not dif-fer between the low-density stand and the high-density stand.seed set rates were significantly lower in the low-density stand than in the high-density stand and experimental cross-pollen supplementation evidenced that foreign pollen availability is indeed a limiting factor in the former.However,seed crops did not differ between the low-density stand and the high-density stand,indicating that limitation of foreign pollen is not likely to be of great concern in terms of reduced seed production and potential recruitment.Poor forest regeneration due to other ecological and human factors is probably a more important threat for the

关 键 词：forest fragmentation genetic diversity paternity analyses pollen dispersal pollen pool structure SELFING tree density 

分 类 号：Q94[生物学—植物学]