苹果脱水素基因生物信息学识别与表达分析  

Bioinformatics Identification and Expression Analysis of Dehydrin Genes in Malus domestica

作　　者：冯闯 王珊珊 赵晨辉[1] 李粤渤[1] 宋宏伟[1] 李红莲[1] 张冰冰[1] 梁英海[1] Feng Chuang;Wang Shanshan;Zhao Chenhui;Li Yuebo;Song Hongwei;Li Honglian;Zhang Bingbing;Liang Yinghai(Research institute of Pomology,Jilin Academy of Agriculture Sciences,Gongzhuling,136100;Faculty of Agronomy,Yanbian University,Yanji,133002)

机构地区：[1]吉林省农业科学院果树研究所,公主岭136100 [2]延边大学农学院,延吉133002

出　　处：《分子植物育种》2022年第4期1112-1118,共7页Molecular Plant Breeding

基　　金：吉林省农业科技创新工程研究生基金(c8223001320)资助。

摘　　要：本研究使用Perl语言在‘金冠’苹果基因组中识别脱水素基因(MD-DHN)序列,并对MD-DHN系统发生关系、保守序列、氨基酸一级结构以及疏水性等进行了生物信息学分析,并利用低温处理下‘金红’、‘金冠’苹果转录组测序数据,分析了MD-DHN表达差异。在‘金冠’苹果基因组中,共识别17个MD-DHN,根据系统发生关系可以归类为I、Ⅱ、Ⅲ3个亚族,I类亚族的成员最多,Ⅲ类亚族最少,均包含Y、S、K序列区,但I类亚族K区一致性不高。17个MD-DHN蛋白都具有高亲水性,但是不同蛋白序列之间亲水性存在差异。17个MD-DHN中,有11个MD-DHN响应苹果冷胁迫和冰冻胁迫,其中10个脱水素基因(MDDHN1~MD-DHN5,MD-DHN8~MD-DHN10,MD-DHN13和MD-DHN15)在冰冻胁迫下,在更耐寒‘金红’苹果中高表达。本研究为进一步探讨MD-DHN基因功能提供了依据。In this study,the gene sequences of dehydrin proteins(MD-DHN)were identified using Perl language in the’Golden Delicious’apple genome,and the phylogenetic relationship,conserved sequence,amino acid primary structure,and hydrophobicity of the MD-DHN genes were analyzed by bioinformatics method.Then,the expression differences of MD-DHN genes were analyzed using the transcriptomic data of’Jinhong’and’Golden Delicious’apples under low temperature stresses,There are 17 MD-DHN genes identified from the’Golden Delicious’apple genome.These genes can be classified into three subfamilies,I,ⅡandⅢaccording to phylogeny relationship.Among these subfamilies,type I include the most members and type andⅢhas the least members,and the three subfamilies contain Y,S,and K sequence regions.However the sequence uniformity in the K region of subfamily I is not very high.The 17 MD-DHN proteins are all highly hydrophilic,but the hydrophilicity of protein sequences are different.Under the low temperature stress,11 MD-DHN responded to chilling and freezing stresses in’Golden Delicious’and’Jinhong’.Among them,the expression levels of 10 dehydrin genes(MD-DHN1~MD-DHN5,MD-DHN8~MD-DHN10,MD-DHN13 and MD-DHN15)in’Jinhong’were significantly higher than those of’Golden Delicious’under the freezing stress.This study provided the important reference for further exploring the function of MD-DHN.

关 键 词：苹果 脱水素 生物信息学 低温胁迫 氨基酸亲水性 

分 类 号：S661.1[农业科学—果树学]