中华猕猴桃蛋白磷酸酶PP2C75基因的生物信息学分析  

Bioinformatics Analysis of Protein Phosphatase PP2C75 Gene from Actinidia chinensis

作　　者：杨彦萍 邓茹友 魏卓 师万源 奚登贤 叶琴霞 张汉尧[2] Yang Yanping;Deng Ruyou;Wei Zhuo;Shi Wanyuan;Xi Dengxian;Ye Qinxia;Zhang Hanyao(Key Laboratory of Biodiversity Conservation in Southwest China,State Forest Administration,Southwest Forestry University,Kunming,6502241;Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming,650224)

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室,昆明650224 [2]西南林业大学西南山地森林资源保育与利用教育部重点实验室,昆明650224

出　　处：《分子植物育种》2022年第4期1119-1126,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31760450);云南省教育厅科学研究基金项目(2018Y131);大学生创新创业训练项目(20180677002)共同资助。

摘　　要：蛋白磷酸酶PP2C是一类重要的信号分子,广泛参与ABA的信号转导途径,包括ABA调控对干旱等逆境胁迫的响应。为了探究PP2C基因在中华猕猴桃这类不耐旱型果树中的功能,本研究使用同源分析法对转录组中的中华猕猴桃蛋白磷酸酶PP2C75基因序列进行分析,利用网上在线分析工具分析其编码蛋白质的结构并预测其功能。研究结果表明,该核苷酸序列的开放阅读框为1206 bp,编码401个氨基酸。其编码蛋白质属于亲水性蛋白,无信号肽,未形成跨膜结构域,具有一个完整且保守的PP2C催化结构域。通过三级结构建模和保守元件比较分析,发现PP2C75与其他响应干旱的PP2C基因有相同的保守元件,相似率达57%,与PP2C16的序列一致度最高,达48.66%。初步推测PP2C75参与ABA的信号转导途径且可能与猕猴桃的干旱胁迫相关。该研究结果可为后续的基因功能确证以及中华猕猴桃品种改良提供参考依据。Protein phosphatase PP2C is an important signal molecule,which is widely involved in the signal transduction pathway of ABA,including response to drought and other stresses.In order to explore the function of PP2C gene in drought-tolerant fruit trees such as Actinidia chinensis,the sequence of the A.chinensis PP2C75gene in the transcriptome was analyzed through the homology analysis method.The physical and chemical properties of PP2C75 protein were analyzed by online analytical tools.At the same time,the protein secondary structure and tertiary structures of protein were predicted.The results of the study indicated that the PP2C75 gene contained an open reading frame of 1206 bp,encoding 401 predicted amino acids.The protein was a hydrophilic protein with no signal peptide,no transmembrane domain,and contained a complete and conserved PP2C catalytic domain.Through tertiary structural modeling and comparative analysis of conservative elements,it was found that PP2C75 of A.chinensis had the same conservative elements as other drought-responsive PP2C genes,with a similarity rate of57%,and the highest consistency with PP2C16 sequence,up to 48.66%.It inferred that PP2C75 gene might play a role in the ABA signal transduction pathway and related to the drought stress in A.chinensis.The results of this study can provide reference for subsequent gene function corroboration and improvement of kiwifruit cultivars.

关 键 词：中华猕猴桃 PP2C75 生物信息学分析 

分 类 号：S663.4[农业科学—果树学]