稻瘟病菌蛋白激酶调控因子MoMOB1的功能分析  

Function Analysis of Protein Kinase Regulator MoMOB1 in Magnaporthe oryzae

作　　者：张君[1] 刘丹 陈培蕊 许庆国 王宗华[1,3,4] 张冬梅 Zhang Jun;Liu Dan;Chen Peirui;Xu Qingguo;Wang Zonghua;Zhang Dongmei(Key Laboratory of Biopesticides and Chemical Biology,(Ministry of Education),College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,350002;State Key Laboratory of Cellular Stress Biology,Xiamen University,Xiamen,361102;Institite of Oceanography,Minjiang University,Fuzhou,350002;Fujian University Key Laboratory for Plant-Microbe Interaction,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]福建农林大学植物保护学院生物农药与化学生物学教育部重点实验室,福州350002 [2]厦门大学细胞应激生物学国家重点实验室和植物遗传重点实验室,厦门361102 [3]闽江学院海洋研究院,福州350002 [4]福建农林大学植物与微生物相互作用福建省高校重点实验室,福州350002

出　　处：《分子植物育种》2022年第4期1170-1175,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31770156)资助。

摘　　要：稻瘟病菌(Magnaporthe oryzae)引起的水稻稻瘟病,每年造成全球水稻减产约10%~30%,对全球粮食生产构成严重威胁。MOB(Mps one binder)蛋白家族成员在物种进化过程中高度保守,并作为重要激酶调控因子与NDR(Nuclear DBF2-related)蛋白激酶等结合,在细胞周期的有丝分裂退出和胞质分离等事件中发挥重要作用。本研究利用功能丧失和获得的方法建立了MoMOB1敲除突变体,对突变体进行了功能回补;并对背景菌株、敲除突变体和回补菌株,在形态特征和致病性等方面进行了生物性状分析,以解析稻瘟病菌中MoMOB1的生物学功能。与背景菌株Ku80相比,MoMOB1缺失后稻瘟病菌生长速率明显减慢,分生孢子萌发延迟,突变体产孢量显著降低,分生孢子出现畸形且隔膜数目异常,部分分生孢子没有隔膜或只有一个隔膜。致病性分析发现,ΔMoMOB1突变体致病性明显减弱。回补菌株的表型与背景菌株Ku80相似。综上所述,MoMOB1在稻瘟病菌营养生长、产孢量、分生孢子发育、附着胞形成和致病性方面发挥关键作用。Rice blast,caused by Magnaporthe oryzae,reduces global rice yield about 10%~30%annually.It is a serious threat to rice production all over the world.Members of the MOB(Mps one binder)protein family are highly conserved in species evolution process.MOB1 as key kinase regulators bind to NDR(Nuclear DBF2-related)protein kinases playing an important role in critical events such as mitotic exit and cytokinesis of cell cycle.In order to elucidate biological function of MoMOB1 in M.oryzae,MoMOB1 gene knock-out mutant and complementary strain were established by loss-and gain-of-function approaches.The strains were analyzed in morphological characteristics and pathogenicity along with background strain Ku80.Comparing to Ku80,the growth rate ofΔMoMOB1 is significantly slowed down and conidia germination lagged.The mutant has a sharp decrease in conidiation and conidia deformity with abnormal septum.Some of the conidia has only one septum or no septum.ΔMoMOB1 mutant decreased in pathogenesity by assays.The phenotype ofΔMoMOB1-Com complementation was similar to that of the background strain Ku80.Taken together,MoMOB1 plays a key role in the vegetative growth,conidiation,conidium development,appressorium formation and pathogenesis in M.oryzae.

关 键 词：稻瘟病菌 MOB1 激酶 致病性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]