93份槭树种质资源的SSR指纹图谱构建与遗传多样性分析  

Construction of SSR Fingerprint and Genetic Diversity Analysis of 93 Maple Germplasm Resources

作　　者：林立[1,2] 林乐静 祝志勇[1] 李波[3] 任杰 Lin Li;Lin Lejing;Zhu Zhiyong;Li Bo;Ren Jie(College of Landscape and Ecology,Ningbo City College of Vocational Technology,Ningbo,315100;College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,210037;Jiangsu Biyuntian Agriculture&Forestry Technology Co.,Ltd.,Danyang,212362;Institute of Agricultural Engineering,Anhui Academy of Agricultural Sciences,Hefei,230001)

机构地区：[1]宁波城市职业技术学院景观生态学院,宁波315100 [2]南京林业大学生物与环境学院,南京210037 [3]江苏碧云天农林科技有限公司,丹阳212362 [4]安徽省农业科学院农业工程研究所,合肥230001

出　　处：《分子植物育种》2022年第4期1250-1263,共14页Molecular Plant Breeding

基　　金：宁波市“科技创新2025”重大专项(2019B10012);丹阳市“丹凤朝阳”人才引进计划项目(2020)共同资助。

摘　　要：槭树为世界名著观赏树种品种丰富,种类繁多。为研究槭树种质资源遗传多样性并建立品种DNA指纹图谱,利用自主开发的15对SSR引物对93份槭树种质进行遗传多样性分析和指纹图谱构建。结果表明,15对SSR引物在93份材料中有效等位基因数(Ne)为2.2071~9.919,平均为6.154;位点多态性信息含量(PIC)在0.449~0.891之间,平均为0.789;观察杂合度(Ho)介于0.013~0.892,平均为0.543;期望杂合度(He)变化范围在0.547~0.899之间,平均为0.812;种群平均Shannon’s信息指数(I)为2.113;遗传多样性指数(H)变幅在0.550~0.904之间,平均为0.817,表明槭树种质资源遗传多样性较高。聚类结果表明,供试槭树品种分为3个类群,与Structure群体遗传结构分析和主坐标分析结果高度一致,并且基于聚类分析和形态鉴定结果,推测‘美峰’为秀丽槭园艺品种。此外,利用15对SSR引物构建了93个槭树品种的DNA指纹图谱。本研究构建的槭树品种资源DNA指纹图谱及遗传分析结果为槭属优良种质资源的鉴别与利用、重要基因的挖掘及遗传育种工作提供了技术支撑。Maple(Acer L.)is a famous ornamental tree species with abundant varieties and various types.In order to assess the genetic diversity of maple germplasm resources and establish DNA fingerprints of maple varieties,15 pairs of SSR primers from 93 were screened out and used to evaluate the genetic diversity of 93 maple germplasms and to construct the variety fingerprints.The results showed that the effective number of alleles(Ne)of 15 SSR primers in 93 maple materials ranged from 2.2071~9.919,with an average of 6.154.The polymorphic information content(PIC)at different locus varied from 0.449~0.891 with an average of 0.789.The observed heterozygosity(Ho)ranged from 0.013~0.892 with an average of 0.543,and the average gene diversity(expected heterozygosity,He)was 0.812,with a range of 0.547~0.899.The average Shannon’s information index(I)and Nei’s gene diversity index(H)were 2.113 and 0.817,respectively.Further,the result of NJ cluster analysis based on genetic distance(GD)showed that 93 maple germplasms were divided into three groups,which was highly consistent with the results of principal coordinate analysis(PCo A)and population genetic structure analysis.Based on the results of cluster analysis and phenotypic identification,it is inferred that Acer palmatum’Oshu Beni’should be revised as A.elegantulum’Oshu Beni’.Additionly,DNA fingerprints of 93 maple varieties were constructed by 15 pairs of SSR primers.In conclusion,maple germplasm resources had a high level of genetic diversity,and abundant genetic variation were observed among different varieties of maple.Therefore,our results of genetic analysis and DNA fingerprinting construction of maple germplasm resources provided technical support for the identification and utilization of maple germplasm resources,the discovering of important genes,and the promoting of genetic breeding.

关 键 词：槭树 SSR分子标记 遗传多样性 指纹图谱 

分 类 号：S792.35[农业科学—林木遗传育种]