百香果连作对土壤真菌群落结构的影响  

Effects on Community Structure of Soil Fungi in Passiflora edulis Continuous Cropping

作　　者：谭仲廷 李安定[2] 杨瑞[1] 蔡国俊[2] 吴凤婵 郭春艳[3] Tan Zhongting;Li Anding;Yang Rui;Cai Guojun;Wu Fengchan;Guo Chunyan(College of Forestry,Guizhou University,Guiyang,550025;Guizhou Institute of Mountain Resources,Guizhou Academy of Sciences,Guiyang,550001;Guizhou Botanical Garden,Guizhou Academy of Sciences,Guiyang,550001)

机构地区：[1]贵州大学林学院,贵阳550025 [2]贵州科学院贵州省山地资源研究所,贵阳550001 [3]贵州科学院贵州省植物园,贵阳550001

出　　处：《分子植物育种》2022年第4期1373-1382,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31960576);贵州省科技计划项目(黔科合支撑[2016]2525号;黔科合支撑[2020]1Y015号;黔科合服企[2019]4004-2;黔科合平台人才[2017]5720-003;黔科合支撑[2020]1Y011号)共同资助。

摘　　要：为了解百香果连作后土壤真菌群落结构的变化,进一步揭示百香果连作障碍发生机制提供依据,本研究采用Illumina MiSeq高通量测序技术,检测及分析百香果连作后土壤真菌群落结构的组成、数量及变化。结果表明:供试土壤样本中共检测到真菌14个门,47个纲,120个目,296个科,612个属,其中子囊菌门(Ascomycota)、担子菌门(Basidiomycotina)是优势菌门;主要的优势属为unclassified_p_Ascomycota属,Saitozyma属,被孢霉属(Mortierella),枝顶孢属(Cladosporium),裸节菌属(Talaromyces),镰刀菌(Fusarium),链格孢属(Alternaria),青霉菌(Penicillium),unclassified_f_Nectricaceae属和附球菌属(Epicoccum);土壤中真菌群落的丰富度指数和多样性指数随百香果连作年限的增加呈上升趋势;属水平上土壤真菌的显著变化可能是造成百香果连作障碍的原因之一;土壤全氮、有机质含量pH是影响土壤真菌群落结构组成的最主要因素。本研究结果可为后期研究百香果连作障碍的生物防治提供研究基础和理论依据。In order to understand the changes of soil fungal community structure after continuous cropping of Passiflora edulis and to provide a basis for revealing the mechanism of continuous cropping obstacles of Passiflora edulis,Illumina Miseq high-throughput sequencing technology was used in this study to detect and analyze the composition,quantity and changes of soil fungal community structure after continuous cropping of Passiflora edulis.The results showed that:A total of 14 phyla,47 classes,120 orders,296 families and 612 genera were detected in the soil samples.Among them,Ascomycota and Basidiomycotina were the dominant phyla.And the dominant genera were unclassified_p_Ascomycota,Saitozyma,Mortierella,Cladosporium,Talaromyces,Fusaria,Alternaria,Penicillium,unclassified_f_Nectricaceae and Epicoccum.The richness indexes and diversity indexes of the fungal community in the soil showed an increasing trend with the increase of the continuous cropping years of Passiflora edulis.At the genus level,the significant change of soil fungi may be one of the reasons for the continuous cropping obstacle of Passiflora edulis.Soil total nitrogen,organic matter content and pH were the main factors affecting the composition of soil fungal community.The results of this study can provide the research basis and theoretical basis for the biological control of continuous cropping obstacle of passion fruit in the later stage.

关 键 词：百香果连作 土壤真菌 群落结构 高通量测序 

分 类 号：S667.9[农业科学—果树学]