Co-Localization of Sampling and Sequencing for Zoonotic Pathogen Identification in the Field Monitoring Using Mobile Laboratories  

作　　者：Xin Lu Yao Peng Yuanyuan Geng Hongqun Zhao Xiaona Shen Dongmei Li Zhenpeng Li Liang Lu Mengguang Fan Wenbin Xu Jin Wang Lianxu Xia Zhongbing Zhang Biao Kan 

机构地区：[1]State Key Laboratory of Infectious Disease Prevention and Control,Beijing,China/National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China [2]General Center for Disease Control and Prevention of Inner Mongolia Autonomous Region,Huhhot City,Inner Mongolia Autonomous Region,China [3]Siziwang Banner Center for Disease Control and Prevention,Huhhot City,Inner Mongolia Autonomous Region,China [4]School of Public Health,Shandong University,Jinan City,Shandong Province,China

出　　处：《China CDC weekly》2022年第12期259-263,I0007,共6页中国疾病预防控制中心周报（英文）

基　　金：Supported by the National Key Research and Development Program of China(2020YFE0205700);the National Science and Technology Major Project(2018ZX10714002);Emergency Response Drill 2021 Project of China CDC.

摘　　要：Introduction:Accurate etiological detection is needed to evaluate the risk of zoonotic diseases.Metagenomic next-generation sequencing(mNGS)can be used to monitor pathogens in animal species and identify potential zoonotic threats.The current sampling model for zoonotic pathogen monitoring in wild animals requires samples to be transferred from the field to a laboratory for further detection.Methods:We constructed a zoonotic pathogen survey model using a set of mobile laboratories.Results:The monitoring in this study was preplanned to detect Yersinia pestis,but the mNGS unexpectedly identified Bartonella spp.in the rodent samples,thus exposing the threat of bartonellosis to humans in this region.The co-localization of sampling and sequencing(CLOSS)model we tested required no long-distance transferring of samples and expands the regional coverage of zoonotic surveys by using a mobile laboratory.Discussion:Using this mNGS technique will enable detection of more zoonotic pathogens beyond the preplanned monitoring targets.This may increase the surveillance efficiency compared with that of the previous workflow and expand the application of the mobile laboratories for infectious diseases identification and surveillance in the field.

关 键 词：enable TRANSFERRED MONITORING 

分 类 号：R535[医药卫生—内科学]