一步原位还原法制备WS_(2)@Au量子点复合物及其传感应用  被引量:1

Facile One⁃Step Synthesis of WS_(2)@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application

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作  者:彭红珍[1,3,4] 张瑜 郭琳洁[1,3] 宋伟 李晴暖[1] 孟祥英 PENG Hong-Zhen;ZHANG Yu;GUO Lin-Jie;SONG Wei;LI Qing-Nuan;MENG Xiang-Ying(Division of Physical Biology,CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China;School of Medical Laboratory,Weifang Medical University,Weifang 261053,China;The Interdisciplinary Research Center,Shanghai Synchrotron Radiation Facility,Zhangjiang Laboratory,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区:[1]中国科学院上海应用物理研究所,中国科学院微观界面物理与探测重点实验室,上海201800 [2]潍坊医学院医学检验学院,潍坊261053 [3]中国科学院上海高等研究院基础交叉研究中心,上海201210 [4]中国科学院大学,北京100049

出  处:《应用化学》2022年第3期480-488,共9页Chinese Journal of Applied Chemistry

基  金:山东省医药卫生科技发展计划项目(No.202012060616);山东省自然科学基金(No.ZR2016BL19)资助

摘  要:电化学传感器界面改造是提升其检测性能的重要途径。其中,增强电化学传感界面的生物相容性和导电性,是电化学传感器发展遇到的一个重大挑战。本文基于一步原位还原法制备的WS_(2)@Au量子点(WS_(2)@Au QDs),对玻碳电极表面进行功能化,用于氧化还原酶的固定,实现了高性能生物传感的构建。借助WS_(2)@Au QDs良好的生物相容性及导电性特性,WS_(2)@Au QDs有效保护了葡萄糖氧化酶(GOx)的催化活性,同时促了进GOx与电极间的直接电子转移,其电子转移速率常数达到2.25 s^(-1)。基于该传感器对葡萄糖良好的电催化作用,该方法被成功用于葡萄糖的检测,其线性范围为5~50μmol/L,检测限为1.5μmol/L,且该传感器具有良好的选择性、重现性和稳定性。WS_(2)@Au QDs在生物传感器的界面改造方面,具有潜在的重要应用前景,其为构建高性能生物传感器奠定了良好的研究基础。The interface modification of electrochemical sensors is an important way to improve their detection performance.Among them,enhancing the biocompatibility and electrical conductivity of the electrochemical sensing interface is a major challenge for the development of electrochemical sensors.In this work,the surface of glassy carbon electrode is functionalized based on WS_(2)@Au quantum dots(WS_(2)@Au QDs)prepared by in situ one-step reduction,which is used to immobilize oxidoreductase,thus realizing the construction of high-performance biosensors.By virtue of the excellent biocompatibility and electrical conductivity of WS_(2)@Au QDs,the WS_(2)@Au QDs can effectively protect the catalytic activity of GOx and promote the direct electron transfer between GOx and the electrode,and the electron transfer rate constant reaches 2.25 s^(-1).Based on the good electrocatalytic effect of the sensor on glucose,the method has been successfully applied to the detection of glucose,with a linear range of 5~50μmol/L and a detection limit of 1.5μmol/L,and the sensor shows good selectivity,reproducibility and stability.These results suggest that the WS_(2)@Au QDs has potential important application prospects in the interface modification of biosensors,which lays a good research foundation for the construction of high-performance biosensors.

关 键 词:WS_(2)@Au 量子点 原位还原 直接电化学 葡萄糖氧化酶 生物传感 

分 类 号:O646[理学—物理化学]

 

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