基于3D打印技术构建石墨烯电化学生物传感器及其应用研究  

Establishment of Graphene Electrochemical Biosensor Based on 3D Printing Technology and Its Application

作　　者：鲁文娟[1] 李阳[2] LU Wenjuan;LI Yang(School of Road,Bridge and Architecture,Shaanxi Railway Institute,Weinan 714000,China;State Key Laboratory of Solidification Processing,College of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China)

机构地区：[1]陕西铁路工程职业技术学院道桥与建筑学院,渭南714000 [2]西北工业大学材料学院,凝固技术国家重点实验室,西安710072

出　　处：《硅酸盐通报》2022年第3期1069-1077,共9页Bulletin of the Chinese Ceramic Society

基　　金：中央高校基本科研业务费专项资金项目(20190202)。

摘　　要：超高灵敏度和特异性的电化学生物传感器在环境风险物质监测以及生物医学检测领域具有重要意义,而构建生物亲和性高、制备工艺简单、成本低廉的检查电极是电化学生物传感器走向应用的关键。本文采用3D打印技术制备出重复性良好的三维石墨烯复合电极,然后通过电化学氧化的方法调控表面石墨烯的形貌和氧化基团。所制备的电化学生物传感器在环境污染物微囊藻毒素(MC-LR)的检测中展现出超高的灵敏度,其线性检测区在4×10^(-6)~1μg/L,检测限为1.5×10^(-7)μg/L。同时,通过改变适配体检测探针后,该电化学生物传感器对于多巴胺、重金属Hg^(2+)、四环素等均具有极高的检测灵敏度。本研究为电化学适配体生物传感器走向应用化提供了一种新的思路,为开发超高灵敏度环境监测和生物医学检测传感器提供一定的基础数据。Electrochemical biosensors with ultra-high sensitivity and specificity are of great significance in the fields of environmental risk substances monitoring and biomedical detection.The key to the application of electrochemical biosensors is to construct a high biocompatibility,simple preparation process and low cost detection electrode.In this paper,three-dimensional graphene electrodes with good reproducibility were prepared by 3D printing technology,and then the morphology and oxidation groups of graphene on the surface were regulated by electrochemical oxidation.The electrochemical biosensor shows high sensitivity in the detection of environmental pollutant microcystin(MC-LR).The linear detection range is 4×10^(-6)μg/L to 1μg/L,and the detection limit is 1.5×10^(-7)μg/L.At the same time,the electrochemical biosensor presents highly sensitivity for dopamine,heavy metal Hg^(2+),tetracycline and so on by changing the aptamer detection probe.This study provides a new idea for the application of electrochemical aptamer biosensors,and provides some basic data for the development of ultra-high sensitivity environmental monitoring and biomedical detection sensors.

关 键 词：3D打印 石墨烯 电化学 生物传感器 超高灵敏度 微囊藻毒素 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]