高通量测序法对糖尿病患者及健康人群粪便微生物的群落分析  

Analysis of fecal microbial community of diabetic patients and healthy people by high-throughput sequencing

作　　者：王梦姣[1] 杨国鹏[1] 郑天骄 李青山 WANG Mengjiao;YANG Guopeng;ZHENG Tianjiao;LI Qingshan(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,Shaanxi,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000

出　　处：《右江医学》2022年第3期167-175,共9页Chinese Youjiang Medical Journal

基　　金：陕西理工大学基金项目(SLG1809)。

摘　　要：目的通过寻找出2型糖尿病患者的粪便指示微生物,为后续进一步开发潜在糖尿病患者试剂盒做准备,并探讨母系亲缘关系与人群粪便微生物多样性的相关性。方法从粪便微生物入手,利用高通量测序法对健康人群及糖尿病患者进行粪便微生物群落的分析和比较。同时,从多样性方面对具有母系遗传关系的四代人类粪便微生物进行比较。结果与健康人群相比,糖尿病人群粪便微生物多样性显著弱于健康人群;厚壁菌门(Firmicutes)、梭菌纲(Clostridia)、梭菌目(Clostridiales),Ruminococcaceae_UCG-013和粪杆菌属(Faecalibacterium)是2型糖尿病患者粪便指示微生物。三相图结果表明,只有布劳特氏菌属(Blautia)连续出现在四代人类粪便微生物群落中;PCoA及LEfSe的差异分析结果表明,四代人粪便微生物群落相似性较差。结论厚壁菌门、梭状芽孢杆菌纲、梭状芽孢杆菌目、Ruminococcaceae_UCG-013和粪杆菌属可作为筛选潜在糖尿病人群的粪便指示微生物。粪便微生物群落与母系遗传关系相关性不强。Objective To prepare for further development of potential diabetic kits through finding out fecal indicator microorganisms of T2DM patients,and to explore the correlation of maternal relationship and fecal microbial diversity.Methods Starting with fecal microorganism,high-throughput sequencing was used to analyze and compare fecal microbial communities in healthy people and T2DM patients.At the same time,four generations of human fecal microorganisms with maternal genetic relationship were compared in terms of diversity.Results Compared with healthy population,the diversity of fecal microorganism in T2DM patients was significantly weaker than that in healthy population.Firmicutes,Clostridia,Clostridiales,Ruminococcaceae_UCG-013 and Faecalibacterium were indicators of fecal microorganisms in T2DM patients.The results of three-phase diagram showed that only Blautia appeared continuously in the four generations of the community of human fecal microorganisms.The difference analysis results of PCoA and LEfSe showed that the similarity of fecal microorganisms communities of the four generations was poor.Conclusion Firmicutes,Clostridia,Clostridiales,Ruminococcaceae_UCG-013 and Faecalibacterium can be used as screening indicators for fecal microorganisms among potential T2DM patients,and correlation between fecal microorganisms community and maternal genetic relationship is not strong.

关 键 词：粪便微生物 2型糖尿病 指示微生物 母系亲缘关系 相关性 

分 类 号：R587.1[医药卫生—内分泌]