呼吸道合胞病毒感染不同气道上皮细胞的病变特点及易感性分析  

Characteristics and Susceptibility of Respiratory Syncytial Virus Infection in Different Airway Epithelial Cells

作　　者：金鑫[1,3] 杨丽 张梅[2,3] 余本莉 尚鲁俊[1] 杨斌 崔玉霞 范丽 余福勋[1,3] 叶芝旭 JIN Xin;YANG Li;ZHANG Mei;YU Benli;SHANG Lujun;YANG Bin;CUI Yuxia;FAN Li;YU Fuxun;YE Zhixu(Guizhou Provincial Peoples Hospital,Guiyang 550002,China;Guizhou Universityy Guiyang 550000,China;Key Laboratory of Pulmonary Immune Diseasesy Chinese Health Commission,Guiyang 550002,China)

机构地区：[1]贵州省人民医院,贵阳550025 [2]贵州大学,贵阳550025 [3]中国卫生健康委员会肺脏免疫性疾病重点实验室,贵阳550025

出　　处：《病毒学报》2022年第2期371-377,共7页Chinese Journal of Virology

基　　金：国家自然科学基金地区基金(项目号:81860003),题目:肠道微生物对RSV感染后PCFs介导的抗病毒免疫应答的影响及机制研究;国家自然科学基金地区基金(项目号:81960001),题目:贵州地区人呼吸道合胞病毒基因变异分析及基因型变迁机制研究;贵州省科技厅支撑计划(项目号:[2018]2785),题目:婴幼儿下呼吸道感染的病毒分离与病因诊断技术研发;贵州省中医药管理局中医药、民族医药科学技术研究课题(项目号:QZYY-2018-010),题目:丹参酮ⅡA磺酸钠体外对RSV复制的影响及机制研究;贵州省卫生计生委科学技术基金项目(项目号:gzwjw2018-1-049),题目:PCFs介导RSV感染后抗病毒免疫应答的机制研究。

摘　　要：为了比较几种不同气道上皮细胞对呼吸道合胞病毒(Respiratory syncytial virus,RSV)感染后病变特点及易感性。采用RSV A2株感染Hep-2、A549、16HBE、BEAS-2B细胞后,奥林巴斯倒置免疫荧光显微镜观察细胞病变效应,免疫荧光检测RSV N蛋白表达及空斑实验测定细胞培养上清液中病毒滴度。结果显示RSV A2株感染Hep-2细胞后最早形成典型的合胞病变,A549细胞次之,16HBE及BEAS-2B形成病变的时间最晚。免疫荧光检测到RSV N蛋白表达特点与细胞病变特点一致。RSV A2在Hep-2上产生的病毒滴度最高,A549细胞次之,在16HBE上产生的病毒滴度最低。提示Hep-2、A549细胞对RSV具有高度易感性,以Hep-2最为敏感。而16HBE、BEAS-2B细胞对RSV感染不敏感,其中16HBE最不敏感。本研究可为选择合适的上皮细胞进行RSV相关研究奠定基础。To compare the pathological characteristics and susceptibility of different airway epithelial cells to Respiratory Syncytial Virus(RSV)infection. Hep-2,A549,16 HBE and BEAS-2B cells were infected with RSV A2 strain. Cytopathic effects were observed by microscopy,RSV N protein expression was detected by immunofluorescence,and virus titer in cell culture supernatant was determined by plaque assay. Results showed that the typical cytopathic effect appeared earliest in HEp-2 cells infected with RSV A2 strain,then in A549 cells,and latest in 16 HBE and BEAS-2B cells. The expression of RSV N protein was consistent with the characteristics of the cytopathic effect. The titer of RSV A2 was highest in Hep-2 cells,followed by A549 cells;the titer was lowest in 16 HBE cells. Our results suggest that Hep-2 and A549 cells are highly susceptible to RSV,and Hep-2 cells are the most sensitive;16 HBE and BEAS-2B cells are not susceptible to RSV,and16 HBE cells are the least susceptible. This study lays a foundation for selecting suitable epithelial cells for RSVrelated research.

关 键 词：呼吸道合胞病毒(RSV) 上皮细胞 细胞病变效应 易感性 病毒滴度 

分 类 号：R373.1[医药卫生—病原生物学]