盐酸胍对口蹄疫病毒完整颗粒和空衣壳的影响  

Effect of Guanidine Hydrochloride on the Intact Particles and Empty Capsids of the Foot-and-mouth Disease Virus

作　　者：陈晶 李昊洲 张韵[2] 孙世琪[2] 方守国[1] 郭慧琛[2] 杨玉莹[1] CHEN Jing;LI Haozhou;ZHANG Yun;SUN Shiqi;FANG Shouguo;GUO Huichen;YANG Yuying(College of Animal Science,Yangtze University,Jingzhou 434025,China;State Key Laboratory of Veterinary Etiological Biology and National Foot-and Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute y Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]长江大学动物科学学院,荆州434025 [2]中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,口蹄疫国家参考实验室,兰州730046

出　　处：《病毒学报》2022年第2期415-421,共7页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:32072859),题目:热休克蛋白HSP60参与口蹄疫病毒形态发生的作用及其机制。

摘　　要：在口蹄疫病毒的复制过程中,会形成完整的病毒粒子(146S)和一定量的空衣壳(75S)。本试验研究了盐酸胍在不同时间和浓度下对口蹄疫病毒146S和75S的影响,并筛选出75S产量最高的条件。通过蔗糖密度梯度离心、透射电镜、特异性单域抗体包被的双抗夹心ELISA等方法对75S和146S进行定性和定量检测。结果表明在口蹄疫病毒(Foot-and-mouth disease virus,FMDV)感染BHK-21细胞2 h时,加入4 mmol/mL的盐酸胍,75S产量比同时间下加入盐酸胍浓度为2 mmol/mL和8 mmol/mL时得到的75S含量高两倍,约为146S的三倍。本研究为定量口蹄疫病毒75S形态的免疫学方法建立奠定了前期研究基础。During foot-and-mouth disease virus(FMDV)replication,complete virus particles(146 S)and a certain number of empty capsids(75 S)are formed. Here,the effect of guanidine hydrochloride on the formation of 146 S and 75 S of the FMDV under different action times and different concentrations was studied,and the conditions for the highest yield of 75 S were screened. Qualitative and quantitative detection of 75 S and 146 S by sucrose density gradient centrifugation,transmission electron microscopy,and heavy-chain antibody-coated double-antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA)were employed. BHK-21 cells were infected by the FMDV for 2 h,upon addition of 4 mmol/mL guanidine hydrochloride,the yield of 75 S was twice as high as the 75 S content obtained when the concentration of guanidine hydrochloride was 2 mmol/mL or 8 mmol/mL at the same action time,and about three-times that of 146 S. This result lays the foundation for use 75 S as a standard product in the DAS-ELISA for quantitative determination of a FMDV-like particle vaccine.

关 键 词：口蹄疫病毒(FMDV) 75S 盐酸胍 

分 类 号：S852.65[农业科学—基础兽医学]