低温常压等离子体提高MDBK细胞中IBRV病毒滴度的作用与机制研究  

Effect of CAP on Titer of Infectious Bovine Rhinotracheitis Virus in Madin-Darby Bovine Kidney Cells,and the Mechanism

作　　者：缪宇杰 韩佩宇 戴晓峰 管政兵[1] MIAO Yujie;HAN Peiyu;DAI Xiaofeng;GUAN Zhengbing(The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China;Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,无锡214122 [2]江南大学无锡医学院,无锡214122

出　　处：《病毒学报》2022年第2期422-430,共9页Chinese Journal of Virology

摘　　要：加强病毒的繁殖可以帮助MDBK细胞生产更多的IBRV病毒用于生产灭活病毒疫苗。已证明低温常压等离子体(Cold atmospheric plasma,CAP)是一种依靠活性氧来实现MDBK细胞中病毒显著增殖的物理方法,可有效增强病毒的传播,其中牛鼻气管炎病毒(Infectious bovine rhinotrachieitis virus,IBRV)和牛肾细胞(Madin-Darby Bovine Kidney,MDBK)分别被用作模型所需病毒和细胞系。CAP被证明与病毒感染具有协同作用,可以在G2/M期阻止宿主细胞分裂增殖,降低细胞膜电位,增加细胞内钙离子水平并诱导细胞选择性自噬。通过检测免疫相关蛋白表达,CAP被证明可以抑制病毒触发的免疫原性信号传导。综上所述,我们证明了CAP触发了宿主资源对病毒繁殖的最大化,这有利于病毒疫苗的生产,并为在医疗和卫生领域应用CAP开辟了新的可能。Enhancing viral reproduction can help with rapid production of vaccines against viral diseases. It has been proven that cold atmospheric plasma(CAP)can effectively enhance the spread of viruses. Here,Infectious Bovine Rhinotracheitis Virus(IBRV)and Madin-Darby bovine kidney cells(MDBK)were used as the virus and cell line in a model to determine the effects of CAP treatment. CAP was shown to have a synergistic effect with the viral infection. It could prevent host cells from dividing and proliferating in the G2/M phase,reduce the cell membrane potential,increase intracellular Ca^(2+) levels,and induce selective autophagy. In addition,by detecting the expression of immune-related proteins,CAP was shown to inhibit virus-triggered immunogenic signaling. In summary,we show that CAP triggers the maximization of host resources for viral reproduction,which is beneficial in the production of vaccines against viruses and in medicine.

关 键 词：低温常压等离子体(CAP) 牛鼻气管炎病毒(IBRV) 牛肾细胞(MDBK) 病毒滴度 

分 类 号：R1[医药卫生—公共卫生与预防医学]