觅食运动对缺血性卒中大鼠慢性应激后抑郁样行为及海马转化生长因子β1表达的影响  

Effects of foraging exercise on depressive-like behaviors and expression of transforming growth factor-β1 in hippocampus of rats with ischemic stroke after chronic stress

作　　者：陶希[1,2] 唐文静 李璐 伍思源 黄丽君 董艺 王婷[1] 秦亮[1] 王淑玲 宋涛[1,2] Tao Xi;Tang Wenjing;Li Lu;Wu Siyuan;Huang Lijun;Dong Yi;Wang Ting;Qin Liang;Wang Shuling;Song Tao(Department of Neurological Rehabilitation,Hunan Provincial People’s Hospital,the First Affiliated Hospital of Hunan Normal University,Changsha 410016,China;Hunan Provincial Key Laboratory of Neurorestoratology,Hunan Normal University,Changsha 410003,China;Hunan Institute of Geriatrics,Hunan Provincial People's Hospital,the First Affiliated Hospital of Hunan Normal University,Changsha 410016,China)

机构地区：[1]湖南省人民医院(湖南师范大学附属第一医院)神经康复科,长沙410016 [2]湖南师范大学神经修复学湖南省重点实验室,长沙410003 [3]湖南省人民医院(湖南师范大学附属第一医院)湖南省老年医学研究所,长沙410016

出　　处：《中华行为医学与脑科学杂志》2022年第1期2-9,共8页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：湖南省自然科学基金项目(2018JJ6104);湖南省卫健委科研计划项目(20200176);湖南省教育厅科学研究项目(20C1172)。

摘　　要：目的:探索觅食运动(foraging exercise,FE)对缺血性卒中大鼠慢性应激后抑郁行为及海马转化生长因子β1(transforming growth factor-β1,TGF-β1)表达的影响。方法:采用线栓法制作大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,将造模成功的30只雄性成年清洁级SD大鼠,根据体质量匹配原则分为卒中组(n=10)和慢性不可预见性温和应激(chronic unpredictable mild stimulation,CUMS)组(n=20)。CUMS组大鼠于造模后1周开始进行3周的CUMS刺激。然后,采用SPSS 24.0软件中的随机数字生成器将抑郁大鼠分为卒中后抑郁(post-stroke depression,PSD)组(PSD组,n=10)和觅食运动组(FE组,n=10)。FE组大鼠进行FE干预,持续4周。分别在MCAO造模后第1周、第4周及第8周结束时进行体质量称量、水迷宫测试、新奇抑制摄食测试(novelty-suppressed feeding test,NSFT)及糖水偏爱实验(sucrose preference test,SPT)。8周后处死大鼠,分别采用免疫组化染色及Western blot检测海马TGF-β1表达变化,ELISA检测血清TGF-β1和TNF-α。采用SPSS 24.0软件进行统计分析,行为学数据的比较采用重复测量方差分析;其他数据多组间比较采用单因素方差分析,进一步两两比较采用LSD检验。结果:(1)大鼠体质量、NSFT潜伏期与摄食量、糖水偏爱率(sucrose preference index,SPI)的组别×时间交互作用显著(F=2.936~12.098,均P<0.05)。4周时,与卒中组[(343.80±19.34)g,(12.10±6.97)s,(0.75±0.09)%]比较,PSD组与FE组体质量[(307.80±17.23)g,(305.30±24.39)g]低、NSFT潜伏期[(21.70±7.02)s,(22.40±8.44)s]长、SPI[(0.52±0.06)%,(0.53±0.07)%]低(均P<0.05);8周时,FE组SPI高于PSD组(P=0.045)。三组体质量、PSD组与FE组NSFT潜伏期、卒中组与FE组NSFT摄食量及PSD组与FE组SPI在不同时间点均差异有统计学意义(F=8.478~196.548,均P<0.05)。水迷宫测试中组别×时间的交互作用不显著。逃避潜伏期的时间主效应(P=0.034)和组别主效应显著(P<0.001):4周时逃避�Objective To explore the effects of foraging exercise(FE)on depressive-like behaviors and expression of transforming growth factor-β1(TGF-β1)in hippocampus of rats with ischemic stroke after chronic stress.Methods The right middle cerebral artery occlusion(MCAO)model was used in 30 male adult clean grade SD rats by suture method.According to the body weight,rats were evenly divided into stroke group(n=10)and chronic unpredictable mild stimulation(CUMS)group(n=20).Rats of CUMS group received stress induction 1 week after operation and lasted for 3 weeks.Then,according to random number generator of SPSS 24.0 software,the depression rats were divided into post-stroke depression(PSD)group(n=10)and FE groups(n=10).The FE group received free FE intervention for 4 weeks.Body weight,water maze test,novelty inhibition feeding test(NSFT)and sucrose preference test(SPT)were performed at the end of the 1st,4th and 8th week,respectively.The expression of TGF-β1 in hippocampus was detected by Immunohistochemistry(IHC)and Western blot(WB),and the levels of TGF-β1 and TNF-αin serum were detected by ELISA.SPSS 24.0 software was used for statistical analysis.The behavioral data were compared by two factor repeated measurement analysis of variance.One way ANOVA was used for comparison among groups,and LSD test was used for further pairwise comparison.Results(1)The interaction between group and time had statistical significance on body weight,latency and food intake of NSFT and sucrose preference index(SPI)(F=2.936-12.098,all P<0.05).After 4 weeks,compared with the stroke group((343.80±19.34)g,(12.10±6.97)s,(0.75±0.09)%),the body weight((307.80±17.23)g,(305.30±24.39)g),and SPI((0.52±0.06)%,(0.53±0.07)%)of PSD group and FE group were lower and the NSFT latency((21.70±7.02)s,(22.40±0.84)s)was longer(all P<0.05).After 8 weeks,SPI in FE group was higher than that in PSD group(P=0.045).There were significant differences in body weight of three groups,NSFT latency and SPI of PSD group and FE group,and food intake of stroke a

关 键 词：觅食运动 卒中后抑郁 慢性应激 海马 转化生长因子Β1 肿瘤坏死因子-α 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R749.4[医药卫生—临床医学]