早熟砂梨‘苏翠1号’与其亲本‘翠冠’‘华酥’成熟果实差异代谢产物及差异基因比较分析  

Comparative Metabolic and Transcriptomic Analysis of Ripening Fruit in Pear Cultivars of’Sucui 1’’Cuiguan’and’Huasu’

作　　者：王宏[1,2] 杨王莉 蔺经[1] 杨青松[1] 李晓刚[1] 盛宝龙[1] 常有宏[1] WANG Hong;YANG Wangli;LIN Jing;YANG Qingsong;LI Xiaogang;SHENG Baolong;CHANG Youhong(Institute of Pomology,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]江苏省农业科学院果树研究所江苏省高效园艺作物遗传改良重点实验室,南京210014 [2]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2022年第3期493-508,共16页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31872078);江苏省梨产业技术体系建设专项资金项目(JATS[2021]436);江苏省农业科技自主创新资金项目[CX(20)2020]。

摘　　要：‘苏翠1号’梨因其极早熟、汁多味甜、肉质细脆而深受广大消费者喜欢。为了解‘苏翠1号’成熟果实主要代谢产物及代谢通路相关基因表达情况,探究其果实优异性状形成的物质和分子基础,对‘苏翠1号’及其亲本‘翠冠’和‘华酥’成熟果实样本进行代谢组和转录组测序分析。代谢组分析发现,3个品种间,前25个差异代谢产物主要包括有机酸、碳水化合物、氨基酸、甘油磷酸脂、脂肪酸和绿原酸。HPLC测定表明,蔗糖含量在‘苏翠1号’中最高,葡萄糖含量在‘翠冠’中最高,果糖含量在‘苏翠1号’和‘翠冠’中相似,略高于‘华酥’,山梨醇含量在‘苏翠1号’和‘华酥’中均显著低于‘翠冠’;苹果酸含量在‘苏翠1号’中最高,柠檬酸含量在‘华酥’中最高,‘苏翠1号’和‘华酥’总酸含量相近,均高于‘翠冠’。氨基酸含量分析表明,‘苏翠1号’的氨基酸总含量及天冬氨酸、苯丙氨酸、甲硫氨酸含量显著高于‘翠冠’和‘华酥’。转录组GO和KEGG分析表明,相较于‘翠冠’和‘华酥’,‘苏翠1号’有机底物分解过程、脂质代谢、有机酸生物合成、羧酸生物合成、多糖代谢等生物学过程及代谢途径显著富集。进一步挖掘差异基因发现,糖代谢中的SPS、山梨醇代谢中的S6PDH、SDH,TCA循环中的FUM1、ICDH、PDH、OGDHL,氨基酸代谢途径中的GAT、GLSN、LASPO、SAMS,以及脂肪酸代谢中的DGK1、ACCA、KASC2、ADH、PLDA等基因在‘苏翠1号’中显著高表达。这些基因可能与‘苏翠1号’更活跃的蔗糖、山梨醇、氨基酸、甘油磷酯代谢及TCA循环有关。An early-maturing pear’Sucui 1’was developed by crossing’Huasu’and’Cuiguan’.The’Sucui 1’produces fruit with sweet taste,crisp texture and low level of stone cells,so it is well accepted by consumers.To explore biochemical and molecular mechanisms that establish the characteristics of the ripening fruit in’Sucui 1’,we carried out the metabolic and transcriptomic analysis using UPLC–MS,HPLC,and an amino acid analyzer and RNA-seq approach.The results from the metabolic analysis showed that metabolites such as sugars,organic acids,amino acids and lipids were significantly different between’Sucui 1’and its parents.Although the total soluble sugar content was similar between’Sucui 1’and’Cuiguan’,and the total organic acid content was similar between’Sucui 1’and’Huasu’,the level of sucrose,malate,Asp,Phe,Met,glycerophospholipids and palmitaldehyde was significantly higher in the ripening fruit of’Sucui 1’.On the other hand,the level of sorbitol and chlorogenic acid was significantly lower in the ripening fruit of’Sucui 1’compared to’Cuiguan’.Differentially expressed genes involved in the biosynthesis pathways of these metabolites were further identified using RNA-Seq.The results from the transcriptomic analysis showed that GO terms such as organic acid biosynthesis,organic substance catabolic process,carbohydrate metabolic process,carboxylic acid biosynthetic process,lipid biosynthetic process and polysaccharide metabolic process were significantly enriched in the comparison of’Sucui 1’to’Cuiguan’and’Huasu’.The higher accumulation of SPS transcripts might be related to the higher level of sucrose in’Sucui 1’.The higher transcript accumulation of S6PDH and SDH might contribute to its more active sorbitol metabolism.Additionally,the upregulation of FUM1,ICDH,PDH,OGDHL might be related to metabolite accumulations in the TCA cycle.Moreover,differential expression of GAT,GLSN,LASPO,SAMS and DGK1,ACCA,KASC2,ADH2 and PLDA might be related to the higher amino

关 键 词：砂梨 果实品质 差异代谢产物 差异基因 qRT-PCR 

分 类 号：S661.2[农业科学—果树学]