高温诱导牡丹产生未减数花粉  

High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa

作　　者：程世平 姚鹏强 耿喜宁 刘春洋 谢丽华 CHENG Shiping;YAO Pengqiang;GENG Xining;LIU Chunyang;XIE Lihua(Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant,School of Chemistry and Environmental Engineering,Pingdingshan University,Pingdingshan,Henan 467000,China)

机构地区：[1]平顶山学院化学与环境工程学院河南省生态经济型木本植物种质创新与利用重点实验室,河南平顶山467000

出　　处：《园艺学报》2022年第3期581-589,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金青年基金项目(31600527);国家自然科学基金河南省联合基金项目(U1804102);平顶山学院高层次人才科研项目(PXY-BSQD2016009)。

摘　　要：以5年生‘凤丹’牡丹为试验材料,在掌握花粉母细胞减数分裂进程的基础上,使用“树木非离体枝芽加热处理装置”(ZL200610113448.X)施加高温处理,诱导花粉染色体加倍,建立了适宜牡丹花粉染色体加倍的技术体系。结果表明,花粉母细胞减数分裂进程和花蕾的外部形态及花药颜色变化有一定相关性,当花蕾处于“小风铃”至“大风铃”时期,即花蕾直径13mm时,花粉母细胞减数分裂达到双线期—中期Ⅰ;此时期40℃高温处理4 h,可获得较高比率(21.98%)具有生活力的2n花粉。In this research,five-year Paeonia suffruticosa’Fengdan’was chosen as an experimental material.On the basis of mastering meiosis process of pollen mother cells,“a non-in-vitro branch bud heat treatment device of trees”(ZL200610113448.X)was used to implement pollen chromosome doubling of P.suffruticosa under high-temperature processing,a suitable technique for high temperature-induced pollen chromosome doubling in P.suffruticosa was established.The findings indicated that meiosis process of pollen mother cells had correlation with the external form of flower buds and anther color changes.When flower buds remained between“small wind bell”stage to“big wind bell”stage,namely the diameter of flower buds was 13 mm,meiosis process of pollen mother cells reached diplonema—metaphaseⅠ.The treatment combination of 40 ℃ for 4 h produced the greatest amount of diploid pollen(up to 21.98% of the total yield).

关 键 词：牡丹 三倍体育种 减数分裂进程 2N花粉 高温 

分 类 号：S685.11[农业科学—观赏园艺]