龙眼GDSL酯酶/脂肪酶基因的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of GDSL Esterase/Lipase Genes in Dimocarpus longan

作　　者：刘梦雨 蒋梦琦 陈燕 张舒婷[1] 薛晓东 肖学宸 赖钟雄[1] 林玉玲[1] LIU Mengyu;JIANG Mengqi;CHEN Yan;ZHANG Shuting;XUE Xiaodong;XIAO Xuechen;LAI Zhongxiong;LIN Yuling(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《园艺学报》2022年第3期597-612,共16页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31672127);福建省自然科学基金项目(2020J01543);福建省高原学科建设经费资助项目(102/71201801101);福建农林大学创新基金项目(CXZX2019033S,CXZX2018078)。

摘　　要：基于第三代龙眼基因组数据库及其转录组数据库,通过生物信息学分析对龙眼GDSL酯酶/脂肪酶家族进行全基因组鉴定。结果显示龙眼GDSL(Dl GDSL)家族共有118个成员,分为15个亚家族,亚细胞定位显示主要分布于细胞外基质中。染色体位置及共线性基因分析表明,118个Dl GDSL基因中有111个定位在15条龙眼染色体上,包括13对共线性基因。基因结构和蛋白质保守基序分析表明该家族的外显子在2~12之间,且motif2和motif3是Dl GDSL家族中尤为重要的保守基序。启动子顺式作用元件分析表明,启动子序列中包含众多光响应、激素应答、无氧诱导、胁迫响应和昼夜节律等响应元件,且大部分成员都含有茉莉酸甲酯(Me JA)、脱落酸(ABA)、生长素(IAA)和赤霉素(GA)等激素应答作用元件。FPKM值分析发现从胚性愈伤组织到球形胚阶段有26个差异表达的基因,提示Dl GDSL家族部分成员可能在龙眼体胚形态建成中发挥重要作用。q RT-PCR分析表明,在外源ABA和Me JA处理下龙眼胚性愈伤组织中,Dl GDSL家族部分成员可能与ABA、Me JA等激素信号转导相关。The evolution characteristics of the longan GDSL(Dl GDSL)family and their expression patterns during the early stages of somatic embryogenesis were investigated.The results showed the 118 members of Dl GDSL family can be divided into 15 subfamilies,and the subcellular localization was mainly distributed in the extracell,a few distributed in other parts such as cell membrane,chloroplast,mitochondria and endoplasmic reticulum.Analysis of chromosomal locations suggests that 111 out of 118 Dl GDSLs are distributed unevenly on the 15 longan chromosomes.Analysis of synteny relationships indicates 13 pairs of Dl GDSL genes.The genomic structures of those Dl GDSL genes indicates that their exon numbers vary between two and twelve.The protein conserved motifs of those Dl GDSL suggest motif2 and motif3 are particularly important.The cis-acting elements of Dl GDSL promoters contained light responsive elements,hormone response,anaerobic induction,stress responsive and circadian control elements.Besides,three family members(A,B and C)of Dl GDSL contains a large number of methyl jasmonate(Me JA),abscisic acid(ABA),auxin(IAA)and gibberellin(GA)action elements.In addition,transcriptome data analysis revealed that 26 differential expression genes from embryogenic callus to globular embryo stage,suggesting members of the Dl GDSL family may play important roles in the morphogenesis of longan somatic embryos.The results of q RT-PCR analysis in different hormone treatments suggests some members of the Dl GDSL family may be related to hormone signal transduction such as ABA and Me JA in longan embryogenic callus.Dl GDSL57/69/105 genes showed obvious up-regulation and Dl GDSL23/42/50/52/75/80/104 genes demonstrated visible down-regulation in ABA treatment.Dl GDSL100 gene revealed obvious up-regulation and Dl GDSL23/27/42/50/52/57/69/80/104/105 showed visible down-regulation in Me JA treatment.In summary,the research will provide a reference for the subsequent functional verification of Dl GDSL genes in somatic embryogenesis.

关 键 词：龙眼 GDSL 全基因组 鉴定 表达分析 

分 类 号：S667.2[农业科学—果树学]