云南大理栽培春兰中ORSV和CymMV的分子检测及序列分析  

Molecular Detection and Sequence Analysis of ORSV and CymMV in Cultivated Cymbidium goeringii in Dali,Yunnan

作　　者：陈健鑫 魏玉倩 唐婕 竺永金 马焕成 伍建榕[1,2] CHEN Jianxin;WEI Yuqian;TANG Jie;ZHU Yongjin;MA Huancheng;WU Jianrong(Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province,College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China;Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration,College of Biodiversity Forestry,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院云南省高校森林灾害预警控制重点实验室,昆明650224 [2]西南林业大学林学院西南地区生物多样性保育国家林业局重点实验室,昆明650224

出　　处：《园艺学报》2022年第3期655-662,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31860208,31560207);国家重点研发计划项目(2019YFD100200X);西南林业大学木棉纤维人工林产业化培育省级创新团队项目(2018HC014)。

摘　　要：2018—2019年,在云南大理部分地区开展了春兰(Cymbidium goeringii)病毒病害的调查和典型植株收集,通过逆转录—聚合酶链式反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)检测春兰中的病毒。70株春兰中均检测到齿兰环斑病毒(odontoglossum ringspot virus,ORSV),未检出建兰花叶病毒(cymbidium mosaic virus,Cym MV),说明该地区潜在的病毒病害主要为ORSV。基于外壳蛋白(CoatProtein)的系统发育树分析表明,所有ORSV分离物位于一个分支。ORSV外壳蛋白属于烟草花叶病毒外壳蛋白家族,理论等电点为5.01,属于亲水性稳定蛋白质。本研究证实了ORSV已经开始感染大理地区栽培的春兰,建议尽快开展兰花病毒病的早期诊断、药剂筛选及防治工作。The survey on virus diseases was carried out and the samples of infected seedlings were collected in Dali,Yunnan,China during 2018—2019.The Reverse Transcription-Polymerase Chain Reaction(RT-PCR)was used to detect the virus.The results showed that total 70 sample were infected by ORSV(odontoglossum ringspot virus).However,Cym MV(cymbidium mosaic virus)was not detected,indicating the major potential infection thread may be from ORSV in Dali.Phylogenetic analysis based on Coat Protein gene(cp)showed that all ORSV clustered into a single clade.The results of protein analysis showed that ORSV coat protein belonged to TMV coat protein family,and the theoretical isoelectric point was 5.01,which was a hydrophilic stable protein.Thus,we confirmed that ORSV had infected in cultivated C.goeringii in Dali.The follow up study should be carried out soon on early diagnosing and screening of anti-virus medicine for orchid viral disease.

关 键 词：春兰 建兰花叶病毒 齿兰环斑病毒 分子检测 外壳蛋白基因 生物信息 

分 类 号：S682.31[农业科学—观赏园艺]