枫香叶斑病病原菌  

Pathogen of Liquidambar formosana leaf spot

作　　者：范绍斌 苏济钰 方甜 潘爱芳 何学友[3] 樊改丽 王宗华[1,5] 胡红莉 FAN Shaobin;SU Jiyu;FANG Tian;PAN Aifang;HE Xueyou;FAN Gaili;WANG Zonghua;HU Hongli(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fuzhou Botanical Garden,Fuzhou 350012,Fujian,China;Fujian Academy of Forestry,Fuzhou 350012,Fujian,China;Xiamen Greening Administration Center,Xiamen 361004,Fujian,China;Institute of Oceanography,Minjiang University,Fuzhou 350108,Fujian,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]福州植物园,福建福州350012 [3]福建省林业科学研究院,福建福州350012 [4]厦门市绿化中心,福建厦门361004 [5]闽江学院海洋研究院,福建福州350108

出　　处：《菌物学报》2022年第3期420-434,共15页Mycosystema

基　　金：国家自然科学基金(31800008);枫香主要病虫害防治研究(闽林科[2016]3号);福建农林大学科技发展及科技创新专项基金项目(CXZX2016137);福建省住房和城乡建设系统2017年第一批科学技术项目(2017-K-90)。

摘　　要：从福建多地采集了55份感染叶斑病的枫香植物病样,包含叶片、叶柄、枝条和树皮。通过分离纯化得到了12个类拟盘多毛孢属真菌菌株,综合形态特征以及3个基因(ITS、β-tubulin和tef1)的分子系统发育分析结果,分别鉴定为Neopestalotiopsis cocoes、N.chrysea、Pestalotiopsis neglecta和P.neolitseae,均为枫香上首次报道,其中N.cocoes在国内首次被发现。通过柯赫氏法则验证,发现N.cocoes能够侵染叶片、叶柄和枝条,N.chrysea能侵染叶片和枝条,P.neglecta能侵染叶柄和枝条,而P.neolitseae不能侵染枫香植物组织。Fifty-five samples of leaves,petioles,branches and bark of Liquidambar formosana infected with leaf spot were collected from several places of Fujian Province,China.Totally 12 isolates of Pestalotiopsis-like fungi were obtained.Based on phylogenetic analysis of ITS,β-tubulin and tef1 genes in combination with morphological characters,the isolates were identified as Neopestalotiopsis cocoes,N.chrysea,Pestalotiopsis neglecta and P.neolitseae.These species are first reported on Liquidambar formosana,among which N.cocoes is first reported in China.The results of Koch’s postulates showed that N.cocoes could infect the leaves,petioles and branches,N.chrysea could infect the leaves and branches and P.neglecta could infect the petioles and branches,but P.neolitseae appeared to be nonpathogenic.

关 键 词：枫香树 叶部病害 类拟盘多毛孢真菌 形态类型 多基因系统发育分析 

分 类 号：S763.7[农业科学—森林保护学]