Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing  

作　　者：Xiaoxuan Lei Liuhanghang Cheng Yu Yang Mengru Pang Yunqing Dong Xuanru Zhu Caihong Chen Zexin Yao Gang Wu Biao Cheng Tymour Forouzanfar 

机构地区：[1]Department of Oral and Maxillofacial Surgery/Pathology,Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA),Vrije Universiteit Amsterdam,Amsterdam Movement Science,Amsterdam,1081HV,The Netherlands [2]Department of Burn and Plastic Surgery,General Hospital of Southern Theater Command,Guangzhou,510030,China [3]Department of Oral Implantology and Prosthetic Dentistry,Academic Center for Dentistry Amsterdam(ACTA),University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU),Amsterdam,1081LA,The Netherlands

出　　处：《Burns & Trauma》2021年第1期72-82,共11页烧伤与创伤（英文）

基　　金：funded by National Natural Science Foundation of China,grant number 81671924,81272105;National Key Research and Development Plan of China,grant number 2017YFC1103301;Health and Medical Treatment Collaborative Innovation Major Special Projects of Guangzhou,grant number 201508020253;Science and Technology Key Project of Guangdong Province,grant number 2014B020212010;Science and Technology Planning Project of Guangdong Province of China,grant number 2015B020233012;Military Medical Innovation Special Projects,grant number 18CXZ029;Zhejiang Provincial Basic Public Welfare Research Project,grant number GJ19H140001;and China’s National Key R&D Programs(NKPs);grant number 2018YFB0407204.

摘　　要：Background:Acute skin wounds may compromise the skin barrier,posing a risk of infection.Small intestinal submucosa(SIS)is widely used to treat acute and chronic wounds.However,the efficacy of SIS to accelerate wound healing still needs to be improved to meet clinical demands.To tackle this problem,platelet-rich plasma(PRP)is used due to its potency to promote proliferation,migration and adhesion of target cells.In this study,we applied PRP and SIS to skin wounds to explore their effects on wound healing by evaluating re-epithelialization,collagen production,angiogenesis and the inflammatory response.Methods:A1×1-cm full-thickness skin defectwas established in mice.Sixty mice were divided into four treatment groups:PRP+SIS,PRP,SIS and control.On days 3,5,7,10 and 14 post-surgery,tissue specimens were harvested.Haematoxylin and eosin,Masson’s trichrome,immunohistochemical and immunofluorescence double staining were used to visualize epidermal thickness,collagen and vascular regeneration and inflammation.Results:Wound contraction in the PRP and PRP+SIS groups was significantly greater,compared with the other groups,on days 3 and 5 post-surgery.A histological analysis showed higher collagen expression in the PRP and PRP+SIS groups on day 7,whichwas associated with a thicker epidermal layer on day 14.In addition,immunohistochemical staining showed that CD31-positive blood vessels and vascular endothelial growth factor expression in the PRP+SIS and PRP groups were significantly higher,compared with the control group.Furthermore,immunofluorescence double staining showed that the number of M1 and M2 macrophages in the PRP+SIS and PRP groups was higher,compared with the control and SIS groups alone,on day 3.However,on day 7,the number of M1 macrophages dramatically decreased in the PRP+SIS and PRP groups.The ratio of M2 to M1 macrophages in the PRP+SIS and PRP groups was 3.97 and 2.93 times that of the control group and 4.56 and 3.37 times that of the SIS group,respectively.Conclusion:Co-administration of SIS and PRP h

关 键 词：Small intestinal submucosa Platelet-rich plasma Full-thickness skin defect M1macrophages M2macrophages Animal model wound healing SKIN 

分 类 号：R68[医药卫生—骨科学]