Sequential temperature control of multi-phasic dormancy release and germination of Paeonia corsica seeds  

作　　者：Marco Porceddu Efisio Mattana Hugh W.Pritchard Gianluigi Bacchetta 

机构地区：[1]Centro Conservazione Biodiversità(CCB),Dipartimento di Scienze della Vita e dell’Ambiente,Universitàdegli Studi di Cagliari.Viale Sant’Ignazio da Laconi,1309123,Cagliari,Italy [2]Royal Botanic Gardens,Kew,Wellcome Trust Millennium Building,Wakehurst Place,Ardingly,West Sussex RH176TN,UK

出　　处：《Journal of Plant Ecology》2016年第4期464-473,共10页植物生态学报（英文版）

基　　金：Sardinia Regional Government:financial support of Marco Porceddu PhD scholarship(P.O.R.Sardegna F.S.E.Operational Programme of the Autonomous Region of Sardinia,European Social Fund 2007-2013-Axis IV Human Resources,Objective l.3,Line of Activity l.3.1.);supported by Ente Foreste della Sardegna;The Royal Botanic Gardens,Kew,receives grant in-aid from Defra,UK.

摘　　要：Aims The physiological responses during dormancy removal and multi-phasic germination were investigated in seeds of Paeonia corsica(Paeoniaceae).Methods Seeds of P.corsica were incubated in the light at a range of temperatures(10-25 and 25/10℃),without any pre-treatment,after W(3 months at 25℃),C(3 months at 5℃)and W+C(3 months at 25℃followed by 3 months at 5℃)stratification,and a GA_(3)treatment(250 mg·l^(−1)in the germination substrate).Embryo growth,time from testa to endosperm rupture and radicle emergence were assessed as separate phases.Epicotyl-plumule emergence was evaluated incubating the germinated seeds at 15℃for 2 weeks,at 5 and 25℃for 2 months on agar water before transplanting to the soil substrate at 10,15 and 20℃and at 15℃for 2 months on the surface agar water with GA_(3).Important Findings Embryo growth,testa rupture,endosperm rupture(radicle emergence)and growth of the epicotyl were identified as four sequential steps in seeds of P.corsica.Gibberellic acid alone and warm stratification followed by 15℃promoted embryo growth and subsequent seed germination.Cold stratification induced secondary dormancy,even when applied after warm stratification.After radicle emergence,epicotyl-plumule emergence was delayed for ca.3 months.Mean time of epicotyl-plumule emergence was positively affected by cold stratification(2 months at 5℃)and GA_(3).P.corsica seeds exhibited differential temperature sensitivity for the four sequential steps in the removal of dormancy and germination processes that resulted in the precise and optimal timing of seedling emergence.

关 键 词：cold/warm stratification embryo growth epicotyl dormancy seed dormancy Paeoniaceae 

分 类 号：Q94[生物学—植物学]