CDMNP-PEG-CD磁纳米颗粒诱导的骨骼肌反应性分析  

Analysis of skeletal muscle reactivity induced by CDMNP-PEG-CD magnetic nanoparticles

作　　者：菅晓婷 王涵 黄静雯 蓝海强 廖钊宏 柯渔[2] 廖华[1] 诸春敏 Jian Xiaoting;Wan Han;Huang Jingwen;Lan Haiqiang;Liao Zhaohong;Ke Yu;Liao Hua;Zhu Chunmin(Guangdong Provincial Key Laboratory of Tissue Construction and Testing,Department of Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515;Department of Biomedical Engineering,School of Life Science and Technology,Jinan University,Guangzhou 510515;Department of Human Anatomy and Tissue and Embryology,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000)

机构地区：[1]广东省组织构建与检测重点实验室,南方医科大学基础医学院解剖教研室,广州510515 [2]暨南大学生命科学与技术学院生物医学工程系,广州510515 [3]兰州大学基础医学院人体解剖与组织胚胎教研室,兰州730000

出　　处：《中国临床解剖学杂志》2022年第2期173-180,共8页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金面上项目(32071181);广东省自然科学基金面上项目(2019A1515011305);广州市科技计划项目(202002030497)。

摘　　要：目的探讨SOMNP、CDMNP及CDMNP-PEG-CD磁性纳米颗粒置入小鼠骨骼肌所诱发的免疫反应性。方法以磁性Fe;O;与SiO;复合制备SOMNP,将聚乙二醇(PEG)、环糊精(β-CD)分子链接SOMNP合成CDMNP,进一步添加聚假单胞烷(polypseudorotaxanes)合成CDMNP-PEG-CD磁性纳米颗粒。分别将3种纳米颗粒置入B6小鼠腓肠肌。组化染色、免疫荧光及FACS分析,分别评估不同置入时段SOMNP、CDMNP和CDMNP-PEG-CD纳米颗粒所诱发的肌毒性及肌内炎性渗出特征。结果SOMNP链接β-CD、PEG/β-CD及polypseudorotaxanes将限制纳米颗粒的肌内扩散,导致CDMNP和CDMNP-PEG-CD在肌内的滞留时间延长、材料邻近区肌细胞坏死、肌内单核/巨噬细胞聚集。较之CDMNP-PEG-CD,CDMNP纳米颗粒吸引更多T细胞进入肌组织。结论SOMNP、CDMNP及CDMNP-PEG-CD纳米颗粒均为潜在的体内免疫诱导物。polypseudorotaxanes修饰赋予CDMNP-PEGCD纳米颗粒较好的体内相容性。Objective To investigate the immunoreactivity induced by SOMNP,CDMNP and CDMNP-PEG-CD magnetic nanoparticles implanted into mouse skeletal muscle.Methods SOMNP was prepared by compounding magnetic Fe;O;and SiO;.Polyethylene glycol(PEG)and cyclodextrin(β-CD)molecules were linked to SOMNP to synthesize CDMNP,and polypseudorotaxanes were further added to synthesize CDMNP-PEG-CD magnetic nanoparticles.particles.Three kinds of nanoparticles were placed into the gastrocnemius muscle of B6 mice,respectively.Histochemical staining,immunofluorescence and FACS analysis were used to evaluate the characteristics of myotoxicity and intramuscular inflammatory exudation induced by SOMNP,CDMNP and CDMNP-PEG-CD nanoparticles at different implantation periods.Results Linking of SOMNP toβ-CD,PEG/β-CD and polypseudorotaxanes will limit the intramuscular diffusion of nanoparticles,resulting in prolonged intramuscular residence time of CDMNP and CDMNP-PEGCD,myocyte necrosis in the vicinity of the material,intramuscular mononuclear/Macrophage aggregation.Compared with CDMNP-PEG-CD,CDMNP nanoparticles attracted more T cells into muscle tissue.Conclusion SOMNP,CDMNP and CDMNP-PEG-CD nanoparticles are all potential immune inducers in vivo.The modification of polypseudorotaxanes endowed CDMNP-PEG-CD nanoparticles with better in vivo compatibility.

关 键 词：磁纳米颗粒 CDMNP-PEG-CD SOMNP CDMNP 免疫反应 骨骼肌 

分 类 号：R318.08[医药卫生—生物医学工程]