褐飞虱对水稻特异性挥发物的行为反应  

Responses of brown planthopper,Nilaparvata lugens(St?l)to specific rice plant volatile organic compounds(VOCs)

作　　者：王晓辉 金奕轩 王艳辉 钟燕妮 李立坤 陈法军[1] WANG Xiao-Hui;JIN Yi-Xuan;WANG Yan-Hui;ZHONG Yan-Ni;LI Li-Kun;CHEN Fa-Jun(Insect-information Ecology Laboratory,Department of Entomology,College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学植物保护学院昆虫系,昆虫信息生态研究组,南京210095

出　　处：《应用昆虫学报》2022年第1期50-57,共8页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(31871963);国家重点研发计划(2017YFD0200400)。

摘　　要：【目的】本研究通过测试褐飞虱Nilaparvata lugens(Stål)对水稻特异性挥发物的行为反应,研究水稻特异性挥发物对褐飞虱选择行为的影响,寻找出对褐飞虱有驱避或引诱功效的水稻挥发物成分,为稻田褐飞虱综合治理提供理论基础。【方法】选择水稻十一烷、十六烷、十七烷、芳樟醇、植醇、2-乙基-1-葵醇、柠檬烯、月桂烯、壬醛、葵醛、2-十二酮、水杨酸甲酯12种挥发物,以正己烷作为溶剂分别配置1、10和100μL/mL 3种浓度处理,采用四臂嗅觉仪测试3种浓度的12种水稻挥发物对褐飞虱行为反应。【结果】发现6种水稻挥发物包括芳樟醇、水杨酸甲酯、植醇、壬醛、葵醛及2-十二酮显著驱避褐飞虱,仅发现月桂烯1种水稻挥发物成分可显著引诱褐飞虱。其中,芳樟醇和水杨酸甲酯在测试的3个浓度中均显著驱避褐飞虱,依照由低到高浓度,褐飞虱对化合物的选择率相较于对照分别下降92.3%、122.2%、242.9%(芳樟醇),57.1%、70.6%、188.9%(水杨酸甲酯),而其它4种驱避剂和1种引诱剂都在浓度达到100μL/mL时才显著影响褐飞虱行为反应。【结论】水稻中存在对褐飞虱具有较好驱避或引诱作用的特异性植物挥发物,可用于生产中开发有效植物源驱避剂或引诱剂用于水稻褐飞虱生态防控。[Objectives]To provide a theoretical basis for the comprehensive management of the brown planthopper,Nilaparvata lugens(Stål),in rice fields by investigating the behavioral responses of this pest to specific rice plant volatile organic compounds(VOCs).[Methods]The behavioral responses of N.lugens to a total of six substances,including alkanes,alkenes,alcohols,aldehydes,ketones and esters,and twelve compounds,including undecane,hexadecane,heptadecane,linalool,phytol,2-hexyl-1-decanol,limonene,myrcene,nonanal,decanal,2-dodecanone and methyl salicylate,in rice plant VOCs,were tested in a four-arm olfactometer at dosages of 1,10 and 100μL/mL with n-hexane as the solvent.[Results]Six VOCs,including linalool,methyl salicylate,phytol,nonanal,decanal and 2-dodecone,were found to be significantly repellant to N.lugens,whereas only one,laurene,was found to be significantly attractive.Linalool and methyl salicylate significantly repelled N.lugens at all three concentrations tested.The repellence of two VOCs increased with dosage;from 92.3%to 122.2%and 242.9%for linalool,and from 57.1%to 70.6%and 188.9%for methyl salicylate.The other four repellents and the single attractant significantly affected the behavior of N.lugens at concentrations of 100μL/mL.[Conclusion]Specific rice plant VOCs are either repellent or attractive to N.lugens,and could therefore potentially be utilized to manufacture effective botanic repellents,or attractants,for the ecological control of N.lugens in rice fields.

关 键 词：水稻 植物挥发物 褐飞虱 驱避或引诱 选择行为反应 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]