水稻雄性不育突变体ms102的鉴定和基因定位  

Identification and Mapping of a Rice Male Sterility Mutant ms102

作　　者：王霞 严维 周志勤 常振仪 郑敏婷 唐晓艳 吴建新 Xia Wang;Wei Yan;Zhiqin Zhou;Zhenyi Chang;Minting Zheng;Xiaoyan Tang;Jianxin Wu(Guangdong Provincial Key Laboratory of Biotechnology for Plant Development/School of Life Sciences,South China Normal University,Guangzhou 510631,China;Shenzhen Institute of Molecular Crop Design,Shenzhen 518107,China)

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州510631 [2]深圳市作物分子设计育种研究院,深圳518107

出　　处：《植物学报》2022年第1期42-55,共14页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.U1901203);广东省自然科学基金(No.2017A030313104)。

摘　　要：水稻(Oryza sativa)隐性核雄性不育突变体是第三代杂交水稻技术的核心。为了挖掘优质雄性不育突变体,该研究通过筛选优质籼稻黄华占(HHZ)的甲基磺酸乙酯(EMS)诱变突变体库,获得1个雄性不育突变体ms102(male sterility mutant 102)。该突变体营养生长正常,但花药不开裂,花粉败育。细胞学分析表明,突变体花药绒毡层不能正常降解,导致小孢子发育异常;遗传分析表明,该突变体的不育表型由1个已报道编码酰基转移酶的DPW2基因突变造成。研究获得了1个隐性核雄性不育突变体,进一步证实了DPW2基因在水稻花药发育中的功能。The recessive nuclear male sterility line is the base of the third-generation hybrid rice breeding technology. To explore new male sterility lines suitable for the breeding technology, we screened an ethyl methylsulfonate(EMS) mutant library derived from the elite xian variety Huanghuazhan(HHZ) and identified male sterility mutant 102(ms102). The ms102 showed a normal vegetative development, but exhibited defective anther dehiscence and pollen abortion. Cytological analyses revealed that the degradation of the tapetum was abnormal in ms102, resulting in the abortion of microspores. Genetic analyses showed that the male sterile phenotype of ms102 was resulted from the mutation of a known male sterility gene Defective Pollen Wall 2(DPW2), which encodes an acyl transferase. Thus, this study identified a recessive nuclear male sterility mutant, and further confirmed the function of DPW2 in rice pollen development.

关 键 词：水稻 雄性不育 基因克隆 

分 类 号：S511[农业科学—作物学]