有氧运动通过调节Lipin1 pre-mRNA选择性剪切和剪接因子SFRS10减轻体重  

Aerobic Exercise Reduces BodyWeight by Regulating Lipin1 Pre-mRNA Alternative Splicing and Splicing Factor SFRS10

作　　者：武宝爱[1] 徐冲 杜会枝[2] WU Bao’ai;XU Chong;DU Huizhi(School of Physical Education,Shanxi University,Taiyuan 030006,China;Institute of Molecular Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学体育学院,山西太原030006 [2]山西大学分子科学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2022年第1期193-199,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31500962)。

摘　　要：通过HE染色、PCR和qPCR等技术研究了有氧运动对小鼠体重、Lipin1 pre-mRNA选择性剪接和剪切因子SFRS10的作用。有氧运动干预后,肥胖模型组小鼠的体重明显下降,Lipin1过表达显著降低。Lipin1有两种剪切变异体:Lipin1α和Lipin1β。肥胖模型组小鼠的Lipin1α表达较低,在有氧运动干预之后升高;而Lipin1β的表达在肥胖模型小鼠组较高,有氧运动干预之后降低。此外,研究发现剪切因子SFRS10参与有氧运动诱导的Lipin1 premRNA选择性剪接。总之,有氧运动可通过调节Lipin1的水平,Lipin1 pre-mRNA的选择性剪切和SFRS10的表达量来减轻体重。The effects of the aerobic exercise on mice body weight,Lipin1 pre-mRNA alternative splicing and SFRS10 by hematoxylin-eosin staining were evaluated by PCR and qPCR techniques.The mice body weight had a significant decrease after the aerobic exercise.The lipin1 over-expressed in obesity group was decreased after the aerobic exercise.One of isoforms,the lipin1αhad a low expression in obesity group and increased after aerobic exercise,while,the other one,the lipin1βhad the opposite change from Lipin1α.Moreover,it was found that SFRS10 was involved in the Lipin1 pre-mRNA alternative splicing induced by the aerobic exercise.In a word,the aerobic exercise may lose the body weight by regulating Lipin1 level,Lipin1 pre-mRNA alternative splicing,and SFRS10.

关 键 词：有氧运动 肥胖 减肥 Lipin1 选择性剪切 SFRS10 

分 类 号：G804.7[文化科学—运动人体科学]