谷子miR169家族及其靶基因的鉴定与功能分析  

Identification and Functional Analysis of mi R169 Family and Its Target Genes in Setaria italica

作　　者：卢平 康庆芳 赵孟瑶 张凤洁[2] 武强强 马芳芳[2] 王宇珅 韩渊怀[2] 王兴春[1] 李雪垠 Lu Ping;Kang Qingfang;Zhao Mengyao;Zhang Fengjie;Wu Qiangqiang;Ma Fangfang;Wang Yushen;Han Yuanhuai;Wang Xingchun;Li Xueyin(College of Life Sciences,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;College of Agriculture,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)

机构地区：[1]山西农业大学生命科学学院,山西晋中030801 [2]山西农业大学农学院,山西晋中030801

出　　处：《作物杂志》2022年第2期54-63,共10页Crops

基　　金：国家重点研发计划(2018YFD1000700,2018YFD1000706);国家自然科学基金(31801434);山西农业大学科技创新基金项目(2017YJ28);山西农业大学科技创新基金项目(2017YJ01);山西省重点研发计划项目(201803D221019-2);山西省应用基础研究项目(201801D221298)。

摘　　要：micro RNA广泛参与植物中多个生长发育过程,mi R169家族是最保守的mi RNA家族之一。对谷子mi R169(sit-miR169)家族进行生物信息学分析,包括染色体分布、系统进化、碱基保守性、二级结构和sit-mi R169及其靶基因的表达。鉴定了15个sit-miR169,分布于7条染色体上。系统进化分析显示,sit-miR169家族前体sit-MIR169大致可以分为2簇,部分sit-MIR169成员与水稻osa-MIR169家族成员亲缘关系较近。sit-miR169的前体均可形成稳定的二级茎环结构,成熟体均产生于前体的5′端臂上,且成熟序列的碱基保守性很强。sit-miR169在花、叶和根中均有表达,具有明显组织表达特异性。sit-miR169的靶基因主要是NF-YA转录因子基因,此外还有L型凝集素类受体蛋白激酶、ω-6脂肪酸去饱和酶、转运蛋白Sec1a、SDG40蛋白、环指跨膜蛋白和核糖体大亚基假尿苷合酶SVR1基因等。mi R169通过调控靶基因,尤其是NF-YA的表达量来影响植物的生长发育和对环境的应答。谷子NF-YA基因主要在根和穗中表达,表现出明显的组织表达特异性。此外,sit-mi R169靶基因的表达受环境诱导,如干旱条件能进一步诱导Seita.9G521100基因在根中的表达。MicroRNA are widely involved in various plant growth and development processes, and the miR169family is one of the most conservative miRNA families. In this study, a series of bioinformatic analyses were conducted on the miR169 family of Setaria italica(sit-mi R169), including chromosomal distribution, phylogeny,base conservativeness, secondary structures, and the expression of sit-miR169 and its target genes. We identified 15 sit-miR169 distributed on seven chromosomes. Phylogenetic analysis showed that the sit-MIR169 family could be mainly divided into two clusters, and some members of sit-MIR169 were closely related to the members of the osa-MIR169 family in rice. All the precursors of sit-miR169 formed a stable secondary stem-loop structure, and the mature ones were all generated from the 5’-end arms of the precursors, and the mature sequences had a strong base conservativeness. The sit-miR169 was expressed in flowers, leaves, and roots, with obvious tissue specificity. The target genes of sit-miR169 were mainly NF-YA transcription factor genes, in addition to L-type lectin-domain containing receptor kinase, ω-6 fatty acid desaturase, protein transporter Secla, protein SDG40, ring finger transmembrane domain-containing protein, and ribosomal large pseudouridine synthase SVR1 genes. The miR169affected plant growth and development and environmental response by regulating the expression of target genes,especially the NF-YA gene. The NF-YA gene in Setaria italica was mainly expressed in roots and spikes, showing obvious specificity tissue expression. In addition, the expression of sit-miR169 target genes was induced by the environmental conditions, for example, drought condition further induced the expression of Seita.9G521100 in roots.

关 键 词：谷子 MIRNA miR169 靶基因 生物信息学 

分 类 号：S515[农业科学—作物学]