光温处理对甘蔗试管苗光合自养生根的影响  

Effects of Light and Temperature on Photoautotrophic Rooting for in Vitro Sugarcane Plantlets

作　　者：刘丽敏 刘红坚 李傲梅 何为中 Liu Limin;Liu Hongjian;Li Aomei;He Weizhong(Sugarcane Research Institute,Guangxi Academy of Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs,Nanning 530007,Guangxi,China)

机构地区：[1]广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/农业农村部广西甘蔗生物技术遗传改良重点实验室,广西南宁530007

出　　处：《作物杂志》2022年第2期153-157,共5页Crops

基　　金：广西创新驱动发展专项资金(桂科AA17202042-11);广西农业科学院基本科研业务专项(桂农科2020YM25,桂发科2021YT008)。

摘　　要：为了提高甘蔗试管苗光合自养生根的成功率,设置了不同季节光温、光补偿和不同光质处理,通过调查无根试管苗生根率、存活率、苗高、根长和单株根数,分析这些因素对无根试管苗生根和生长的影响。结果表明,冬季低温弱光照时期甘蔗试管苗光合自养生根的生根率和存活率比夏季高温强光照时降低。冬季光补偿可以促进无根试管苗植株生长和根系伸长,但对试管苗的生根率和存活率没有显著影响。与自然光处理相比,单独红光和蓝光处理不利于甘蔗试管苗的生根与生长,而自然光补充蓝光或红光均能促进试管苗增高及根伸长,对试管苗的生根率和存活率没有显著影响。To improve the rate of photoautotrophic rooting for in vitro sugarcane plantlets under artificial planting condition, treatments with different light and temperature compensation and light quality were carried out. The effects of these factors on rooting and growth were analyzed through characters of rooting rate, survival rate,seedling height, root length and root number per plant for rootless seedlings. The results showed that the rooting rate and survival rate for photosynthetic autotrophic rooting of sugarcane in vitro plantlets decreased in winter due to the low temperature and low light compared with the summer. In winter light supplementation improved the photosynthetic autotrophic rooting length and seedling growth for in vitro plantlets, but there was no significant effect on the rooting rate and survival rate. Compared with the natural light treatment, pure irradiation of red light and blue light treatments were not conducive to the rooting and growth of sugarcane in vitro plantlets, while natural light supplemented by blue light and red light could promote the seedling height and root elongation for in vitro plantlets, but there was no significant effect on the rooting rate and survival rate of in vitro plantlets.

关 键 词：甘蔗 试管苗 光温 光合自养生根 

分 类 号：S566.1[农业科学—作物学]