血清(1,3)-β-D-葡聚糖测定主试剂溶解方式不同对检测结果的影响  

Effect of different dissolution methods of main reagents in serum(1,3)-β-D-glucan determination(G test)on the detection results

作　　者：黄日昇 彭云娟 李轶春 HUANG Ri-sheng;PENG Yun-juan;LI Yi-chun(Department of Medical Laboratory,Beihai Hospital of Traditional Chinese Medicine,Beihai 536000,Guangxi,CHINA)

机构地区：[1]北海市中医医院医学检验科,广西北海536000

出　　处：《海南医学》2022年第7期868-871,共4页Hainan Medical Journal

摘　　要：目的探讨血清(1,3)-β-D-葡聚糖[(1,3)-β-D-glucan,BDG]测定(G试验)反应主试剂溶解方式不同对检测结果的影响。方法选取2019年6~12月北海市中医医院重症医学科、肺病科及肿瘤科拟诊侵袭性真菌感染(IFI)住院患者126例,按照IFI确诊标准分成感染组60例和未感染组66例,比较两组G试验反应主试剂手工振荡溶解与仪器振荡溶解实验检测的标准差、敏感度、特异度、室内质控数据分析及仪器振荡溶解时间推测,统计学分析两种溶解方式不同对检测结果的影响。结果两组患者的G试验反应主试剂仪器振荡溶解方法较手工振荡溶解方法有较低的标准差,且两组两种溶解方法检测结果比较差异均有统计学意义(P<0.05);感染组反应主试剂手工振荡溶解方法与仪器振荡溶解方法检测结果的敏感度分别为81.7%、90.0%;未感染组两种溶解方法的特异度分别为84.8%、92.4%,差异有统计学意义(P<0.05);随机挑选112 d室内质控数据分析,反应主试剂手工振荡溶解与仪器振荡溶解方法失控率分别为8.930%和0.890%,差异有统计学意义(P<0.05);随机选择30 d室内质控统计,手工振荡溶解与仪器振荡溶解方法均值分别为(241.41±47.35)pg/mL和(209.37±8.97)pg/m L,差异有统计学意义(P<0.05);仪器振荡溶解10 s与20 s以上两种方法检测结果,室内质控统计数值均值分别为(209.88±1.65)pg/m L和(209.27±1.33)pg/m L,差异无统计学意义(P>0.05)。结论G试验(光度法)反应主试剂仪器振荡(振荡10 s即可)较手工振荡溶解方法能降低标准差,提高敏感度与特异度。Objective To explore the influence of different dissolution methods of main reagents in serum(1,3)-β-D-glucan[(1,3)-β-D-Glucan,BDG]determination(G test)on the detection results.Methods A total of 126 inpatients with invasive fungal infection(IFI)in the Department of Critical Care Medicine,Department of Pulmonary Diseases,and Department of Oncology at Beihai Hospital of Traditional Chinese Medicine were selected from June to December,2019.They were divided into 60 patients in the infected group,and 66 patients in the uninfected group according to the IFI diagnosis standard.The standard deviation,sensitivity,specificity,analysis of indoor quality control data and speculation of instrument oscillation dissolution time of main reagent manual oscillation dissolution and instrument oscillation dissolution test in G test reaction were compared between the two groups.Effects of different dissolution methods on the detection results were statistically analyzed.Results The standard deviation of instrument oscillation dissolution method for G test reaction main reagent in the two groups was lower than that of manual oscillation dissolution method,and the difference between the detection results of two dissolution methods in the two groups was statistically significant(P<0.05).The sensitivity of the detection results of the main reagent by manual oscillation dissolution method and instrument oscillation dissolution method in the infected group were 81.7%and 90.0%,respectively.The specificity of two dissolution methods in uninfected group was 84.8%and 92.4%,respectively,and the difference was statistically significant(P<0.05).The randomly selected 112-day indoor quality control data analysis showed that the out-of-control rates of manual oscillation dissolution and instrument oscillation dissolution of main reagents were 8.930%and 0.890%,respectively,and the difference was statistically significant(P<0.05).According to the random statistics of 30 days’indoor quality control,the mean values of manual shaking dissolution an

关 键 词：血清(1 3)-β-D-葡聚糖 溶解方式 侵袭性真菌感染 反应主试剂 G试验 

分 类 号：R446.1[医药卫生—诊断学]