冰石长皮软膏拆方对皮肤慢性溃疡大鼠胶原沉淀、炎症因子及VEGF蛋白水平的影响  

Effects of Bingshi Changpi Ointment on Collagen Deposition,Inflammatory Factors and Vegf Protein Levels in Rats with Chronic Skin Ulcer

作　　者：王蕾[1] 李琰[1] WANG Lei;LI Yan(Department of Traditional Chinese Medicine,the Ninth People's Hospital Affiliated to the Medical College of Shanghai Jiaotong University,Shanghai 200125,China)

机构地区：[1]上海交通大学医学院附属第九人民医院中医科,上海200125

出　　处：《中国美容医学》2022年第3期90-95,共6页Chinese Journal of Aesthetic Medicine

基　　金：上海市卫生健康委员会中医药科研项目—冰石长皮软膏拆方治疗下肢静脉性溃疡的临床评价及微生态调控机制研究(编号:2020LP002)。

摘　　要：目的:探究冰石长皮软膏拆方对皮肤慢性溃疡大鼠胶原沉淀、炎症因子及VEGF蛋白水平的影响。方法:把实验所用的75只小鼠随机分为5组(n=15),分别为空白组、模型组、原方组、拆方组、西药组,空白组造普通溃疡创面模型,其余4组造慢性溃疡创面模型。空白组和模型组大鼠创面敷生理盐水纱布条,原方组大鼠创面敷冰石长皮软膏原方药膏纱布条,拆方组大鼠创面敷冰石长皮软膏拆方药膏纱布条,西药组大鼠创面敷凡士林油纱布条。观察各组大鼠一般状态,创面面积及愈合率,HE染色比较各组大鼠创面组织形态变化,ELISA检测比较各组大鼠创面血清中炎症因子IL-8和TNF-α的含量,蛋白质印迹及RT-PCR检测各组大鼠组织中VEGF蛋白和mRNA的表达。结果:在造模后,各组大鼠一般情况变差;用药时,大鼠创面颜色变深,肉芽组织相对减少。造模后,创面面积模型组<原方组<拆方组<西药组,但愈合率原方组>拆方组>西药组>模型组,差异有统计学意义(P<0.05)。和模型组大鼠创面组织病理学比较,西药组、拆方组和原方组大鼠组织的创面和炎细胞浸润逐渐减少,而组织中毛细血管的数量逐渐增多。给药3、7d时,西药组、拆方组和原方组创面炎症因子IL-8和TNF-α的含量均低于模型组(P<0.05),且呈逐渐递减趋势。VEGF蛋白和mRNA结果显示,西药组、拆方组和原方组大鼠创面组织中VEGF蛋白和mRNA表达均显著高于模型组(P<0.05),且呈逐渐递增趋势。结论:冰石长皮软膏拆方可有效增加慢性皮肤溃疡胶原沉淀,促进创面愈合,减轻创面组织中炎症因子的含量及促进血管内皮生长因子VEGF的表达。Objective To explore the effects of Bingshi Changpi ointment on collagen precipitation,inflammatory factors and VEGF protein levels in rats with chronic skin ulcer.Methods The 75 mice used in the experiment were randomly divided into 5 groups(n=15),which were blank group,model group,original formula group,disassembled formula group and Western medicine group.The blank group made ordinary ulcer wound model,and the other 4 groups made chronic ulcer wound model.Rats in the blank group and the model group were applied with normal saline gauze strips,rats in the original formula group were applied with Bingshi Changpi ointment,rats in the simplified group were applied with Bingshi Changpi ointment,rats in the western medicine group were applied with vaseline oil gauze strips.The general state,wound area and healing rate of rats in each group were observed.The morphological changes of wound tissue in each group were compared by HE staining,and the inflammatory factors IL-8 and TNF in wound serum of rats in each group were detected by ELISA-αThe expression of VEGF protein and mRNA in rat tissues was detected by Western blot and RT-PCR.Results After modeling,the general condition of the rats in each group became worse;when the drug was administered,the color of the wound surface of the rats became darker,and the granulation tissue was relatively reduced.After modeling,the wound area model group<original recipe group<disassembled recipe group<western medicine group,but the healing rate of the original recipe group>disassembled recipe group>western medicine group>model group,the difference was statistically significant(P<0.05).Compared with the histopathology of the wounds of the rats in the model group,the wounds and inflammatory cell infiltration in the tissues of the western medicine group,the disassembled prescription group and the original prescription group gradually decreased,while the number of capillaries in the tissues gradually increased.On the 3rd and 7th day of administration,the contents of inflammatory fact

关 键 词：冰石长皮软膏拆方 皮肤 慢性溃疡 胶原沉淀 创面愈合 炎症因子 VEGF 

分 类 号：R632.1[医药卫生—外科学]