冷驯化下中缅树鼩BAT中主要内分泌信号通路转录组分析  

Transcriptome analysis of major endocrine signaling pathways of BAT in Tupaia belangeri during cold acclimation

作　　者：王慧娟 贾婷 朱万龙[1] 王政昆[1] WANG Huijuan;JIA Ting;ZHU Wanlong;WANG Zhengkun(Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College,Engineering Research Center of Ministry of Education for Sustainable Development and Utilization of Bioenergy,Key Laboratory of Biomass Energy and Environmental Biotechnology in Yunnan Province,School of Life Sciences,Yunnan Normal University,Kunming 650500,China;Medical college,Yunnan University of Business Management,Kunming 650106,China)

机构地区：[1]云南师范大学,生命科学学院,云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,生物能源持续开发利用教育部工程研究中心,云南省生物质能与环境生物技术重点实验室,昆明650500 [2]云南经济管理学院医学院,昆明650106

出　　处：《生物学杂志》2022年第2期45-51,共7页Journal of Biology

基　　金：国家自然科学基金项目(31660121);云南省中青学术和技术带头人后备人才项目(2019HB013);云南省万人计划青年拔尖人才项目(YNWR-QNRC-2019-047)。

摘　　要：为探究冷驯化条件下,胰岛素和甲状腺激素信号通路在中缅树鼩褐色脂肪组织(brown adipose tissue,BAT)中的调节机制,利用Illumina novaseq 6000平台对对照组和冷驯化组中缅树鼩BAT进行转录组测序,通过生物信息学分析筛选出与胰岛素和甲状腺激素相关的信号通路及候选基因。结果显示:冷驯化组较对照组,共2879个基因表达存在显著差异,其中1181个上调,1698个下调;GO分析结果显示细胞组分、分子功能、生物学过程分别显著富集到24、28和55条term,差异表达基因主要富集在生物学过程;KEGG分析差异表达基因显著富集到20条pathways,其中筛选到与胰岛素和甲状腺激素信号通路相关的候选基因有PDPK1、AKT2、ACACA、SLC2A4和THRB等。结果表明,中缅树鼩可通过调节BAT中胰岛素和甲状腺激素信号通路中基因表达来应对寒冷环境。In order to investigate the regulation mechanism of insulin and thyroid hormone signaling pathways in brown adipose tissue(BAT)in Tupaia belangeri under cold acclimation,in the present study,transcriptome sequencing of BAT in T.belangeri between control and cold acclimation group were carried out by Illumina novaseq 6000 platform,respectively.Then the signal pathways and candidate genes related to insulin and thyroid hormone were screened out by bioinformatics analysis.The results showed that there were significant differences in the expression of 2879 genes in cold acclimation group compared with that of control group,1181 were up-regulated and 1698 were down-regulated in cold acclimation group.The results of GO analysis showed that there were 24,28 and 55 terms were significantly enriched in cellular components,molecular functions and biological processes,respectively,and differentially expressed genes were mainly enriched in biological processes.KEGG analysis showed that the differentially expressed genes were significantly enriched in 20 pathways,and candidate genes related to insulin and thyroid hormone signaling pathways were screened out,including PDPK1,AKT2,ACACA,SLC2A4,and THRB,etc.All of the above results indicated that the T.belangeri could adapt to the cold environment by regulating genes expression in insulin and thyroid hormone pathways in BAT.

关 键 词：转录组 中缅树鼩 褐色脂肪组织 胰岛素信号通路 甲状腺激素信号通路 

分 类 号：Q494[生物学—生理学] Q953