一株自生固氮菌的筛选、鉴定及生长条件优化  

A strain of abiogenousazotobacter:screening,identification and optimization of growth conditions

作　　者：汪蕊露 李进[1] 王翀[1] 刘虎虎[1] 卢向阳[1] 田云[1] WANG Ruilu;LI Jin;WANG Chong;LIU Huhu;LU Xiangyang;TIAN Yun(College of Biological Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128

出　　处：《生物学杂志》2022年第2期69-73,79,共6页Journal of Biology

基　　金：湖南省科技重大专项(2018NK1030);湖南省科技特派员创新创业项目(2020NK4194);湖南战略性新兴产业科技攻关与重大科技成果转化项目(2019GK4018);湖南农业大学“双一流”建设项目(SYL201802002)。

摘　　要：为筛选获得稳定高效的自生固氮菌,从多个地区植物根际土壤以及淤泥中选取样品,以无氮培养基进行分离筛选,采用乙炔还原法测定固氮酶活性,经形态学观察、生理生化试验以及16S rDNA序列分子鉴定,并通过单因素与响应面试验相结合的方式对生长条件进行优化。结果表明,从山西省朔州市玉米根际的土壤中筛选到一株固氮酶活性达140.14 nmol C_(2)H_(4)/mg·protein·hr的自生固氮菌,该菌鉴定为Azotobacter属,命名Azotobacter sp.strain C-5-2;菌株最适生长的条件为蔗糖浓度10 g/L、转速148 r/min、温度32℃以及初始pH 9.0。In order to screen and obtain stable and efficient abiogenousazotobacter,samples were selected from the soil of plant rhizosphere and silt in multiple regions,separated and screened with nitrogen-free medium,and nitrogenase activity was determined by acetylene reduction method.Morphological observation,physiological and biochemical test and 16S rDNA sequence molecular identification were made.Furthermore,the growth conditions were optimized through a combination of single factor and response surface test.The results showed that a strain of abiogenousazotobacter with nitrogenase activity of 140.14 nmol C_(2)H_(4)/mg·protein·hr was screened from the soil of the corn rhizosphere in Shuozhou,Shanxi Province.It was identified as Azotobacter and named Azotobacter sp.strain C-5-2;the optimal conditions for the growth of the strain were sucrose concentration 10 g/L,rotation speed 148 r/min,temperature 32℃and initial pH 9.0.

关 键 词：自生固氮菌 筛选 生长条件 响应面优化 

分 类 号：Q93-331[生物学—微生物学]