清醒小鼠背侧耳蜗核单个神经元的细胞外记录技术  

Single-neuron recordings in dorsal cochlear nucleus of awake mouse

作　　者：秦春晖 汤正权 QIN Chunhui;TANG Zhengquan(School of life science,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学生命科学学院,合肥230601

出　　处：《生物学杂志》2022年第2期106-109,共4页Journal of Biology

基　　金：国家自然科学基金面上项目(82071061);安徽省留学回国人员创新创业项目(2020LCX036)。

摘　　要：为建立对清醒动物深层脑区进行单个神经元的细胞外电生理记录的方法,采用玻璃微电极和头部固定系统对清醒小鼠背侧耳蜗核的主神经元作急性细胞外的电信号记录,通过观察电极记录的神经元对声音刺激反应进一步确定是否是听觉背侧耳蜗核神经元。在体单细胞记录研究结果表明:主神经元梭形细胞对纯音刺激诱导反应的频率调谐曲线呈“V”型,刺激时间直方图为“建立型”,并且对动物发声有选择性地反应。在体单细胞记录的主神经元梭形细胞在静息状态下进行自发放电活动,此现象与脑片膜片钳技术记录结果一致。此外,研究结果表明梭形细胞的电生理活动与其他中间神经元有明显的差别:车轮细胞在静息电位下具有暴发式放电活动,垂直细胞在静息状态下无自发放电活动。研究结果表明此方法可以准确鉴定深层脑区不同神经元细胞类型。To explore the extracellular recordings of single unit in the deep brain regions of awake mice,we recorded the neural activity of dorsal cochlear nucleus in awake head-fixed mice using glass micropipettes,and compared with that using brain-slice patch-clamp recordings.The extracellular single-cell recording results showed that principal neurons in dorsal cochlear nucleus were identified:fusiform cells showed spontaneous spike discharge activity at resting potential,“V”shape of sound-induced response frequency tuning curve(FTC),buildup response of post-stimulus time histogram(PSTH),and selectively response to sound vocalizations.Consistent with the results obtained using brain-slice patch-clamp recordings,fusiform cells identified by this extracellular single-unit recording showed spontaneous discharge activity.The neural activity of fusiform cells at rest was different from those of two classes of interneurons in the dorsal cochlear nucleus:cartwheel cells fired spontaneously a burst of spikes at rest;vertical cells remained silent at rest.Therefore,this study suggested that this extracellular single unit recording could be used to identify different cell types in deep brain regions in vivo.

关 键 词：单细胞细胞外记录 背侧耳蜗核 清醒小鼠 自发放电活动 细胞类型 

分 类 号：Q42[生物学—神经生物学] R338[生物学—生理学]