一株野生硫磺菌的分类鉴定及其菌丝培养条件优化  

Identification and optimization of mycelium culture condition of a wild Laetiporus sulphureus stain

作　　者：李艳婷 郭尚 郎慧芳 郭霄飞 陈楠 Li Yanting;Guo Shang;Lang Huifang;Guo Xiaofei;Chen Nan(Shanxi Agricultural University,Shanxi Institute for Functional Food,Taiyuan,Shanxi 030031,China;Agriculture and Animal Husbandry Service Center of Mount Wutai Scenic Area,Xinzhou,Shanxi 034000,China)

机构地区：[1]山西农业大学山西功能食品研究院,山西太原030031 [2]五台山风景区农业和畜牧业服务中心,山西忻州034000

出　　处：《食药用菌》2022年第2期138-143,共6页Edible and Medicinal Mushrooms

基　　金：山西省科技成果转化引导专项项目(201904D131042);山西省农业科学院科研创新团队专项(YGC2019TD03);山西省农业科学院农业科技创新研究课题(YCX2020SJ04)。

摘　　要：经形态学和分子生物学鉴定,明确采自山西的一株野生大型真菌为硫磺菌,对其进行分类鉴定,并采取单因素试验研究碳源、氮源、碳氮比、培养温度、pH对该菌菌丝体生长的影响。结果表明:该菌菌丝体生长的最佳碳源为葡萄糖,最佳氮源为酵母膏;通过响应面优化得到菌丝体最佳培养条件为碳氮比40∶1,培养温度26.5℃,pH 5,在此条件下菌丝生长速率为5.608 mm/d。A wild Laetiporus sulphureus collected from Shanxi was classified and identified.The effects of carbon source,nitrogen source,carbon nitrogen ratio,culture temperature and pH on the mycelial growth of the fungus were analyzed by single factor test,and the mycelial culture conditions were optimized by response surface test.The results indicated that the best carbon source for mycelium growth was glucose and the best nitrogen source was yeast extract.Through response surface optimization,the optimum culture conditions of mycelium were C/N ratio was 40∶1,culture temperature was 26.5℃,pH 5.Under these conditions,the mycelium growth rate of Laetiporus sulphureus was 5.608 mm/d.

关 键 词：硫磺菌 鉴定 菌丝 培养条件优化 

分 类 号：S646[农业科学—蔬菜学] S567.3[农业科学—园艺学]