醛糖还原酶抑制剂抑制屋尘螨诱导的人支气管上皮细胞系BEAS-2氧化应激反应  

Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite

作　　者：鲍敏 林玫 甄海宁 何芳 丁敏 袁竹青 陈亚隽 薛欣欣 BAO Min;LIN Mei;ZHEN Hai-ning;HE Fang;DING Min;YUAN Zhu-qing;CHEN Ya-jun;XUE Xin-xin(Department of Respiratory and Critical Care Medicine, Wuhan Third Hospital,Wuhan 430060, China)

机构地区：[1]武汉市第三医院呼吸与危重症医学科,湖北武汉430060

出　　处：《基础医学与临床》2022年第4期616-621,共6页Basic and Clinical Medicine

基　　金：武汉市卫生健康委员会科研课题(WX19C06)。

摘　　要：目的探究醛糖还原酶抑制剂ARIs抑制屋尘螨诱导的人支气管上皮细胞系BEAS-2B氧化应激反应。方法将BEAS-2B分为对照组、模型组、NF-κB抑制剂组(PDTC组)和ARIs干预组(ARIs组)。检测氧化应激指标(MDA和ROS含量、SOD活性);ELISA检测IL-5、IL-13、IFN-γ含量;MTT法检测细胞存活率;流式细胞仪检测细胞凋亡;蛋白质印迹检测细胞内NF-κB、NF-κB p65和p38 MAPK蛋白表达。结果模型组细胞中SOD活性、IFN-γ含量及细胞存活率均低于对照组(P<0.05),MDA、IL-5、IL-13含量及细胞凋亡率高于对照组(P<0.05);与模型组相比,PDTC组和ARIs组细胞中SOD活性、IFN-γ含量及细胞存活率升高(P<0.05),MDA、IL-5、IL-13含量及细胞凋亡率降低(P<0.05);和对照组相比,模型组细胞中NF-κB、NF-κB p65和p38 MAPK蛋白表达均显著增加(P<0.05);和模型组相比,PDTC组和ARIs组细胞中NF-κB、NF-κB p65和p38 MAPK蛋白表达均明显降低(P<0.05)。结论醛糖还原酶抑制剂可改善屋尘螨诱导的BEAS-2B发生的氧化应激反应,促进BEAS-2B细胞增殖,抑制凋亡。Objective To investigate the inhibition of house dust mite induced oxidative stress with human bronchial epithelial cell line BEAS-2B by aldose reductase inhibitor(ARIs).Methods BEAS-2B was divided into control group,model group,NF-κB inhibitor group(PDTC group)and ARIs intervention group(ARIs group).Oxidative stress reaction(MDA and ROS content,SOD activity)was detected by ELISA.The contents of IL-5,IL-13 and IFN-γwere measured by ELISA.MTT was used to detect cell viability.Cell apoptosis was detected by flow cytometry.The expressions of NF-κB,NF-κB P65 and P38 MAPK proteins were detected by Western blot.Results SOD activity,IFN-γcontent and cell survival rate in model group were lower than those in control group(P<0.05),MDA,IL-5,IL-13 content and cell apoptosis rate were higher than those in control group(P<0.05).Compared with model group,SOD activity,IFN-γcontent and cell survival rate in PDTC and ARIs groups wereincreased(P<0.05),MDA,IL-5,IL-13 content and cell apoptosis rate were decreased(P<0.05).Compared with control group,the expression of NF-κB,NF-κB P65 and P38 MAPK protein in model group was significantly increased(P<0.05).The expression of NF-κB,NF-κB P65 and P38 MAPK protein in PDTC group and ARIs group were significantly decreased(P<0.05).Conclusions Aldose reductase inhibitors improve the oxidative stress response of human bronchial epithelial cells induced by house dust mites,promote the proliferation of BEAS-2B cells and inhibit apoptosis.

关 键 词：醛糖还原酶抑制剂 人支气管上皮细胞 氧化应激 屋尘螨 

分 类 号：R562.2[医药卫生—呼吸系统]