氯胺酮减轻大鼠脊髓缺血/再灌注损伤  

Ketamine alleviates spinal cord ischemia-reperfusion injury of rats

作　　者：陈勇[1] 庞红利[1] 许远征[1] 毛珊珊 洪道先[1] CHEN Yong;PANG Hong-li;XU Yuan-zheng;MAO Shan-shan;HONG Dao-xian(Department of Anesthesiology and Perioperative Medicine, the First Affiliated Hospital of Henan University, Kaifeng 475001, China)

机构地区：[1]河南大学第一附属医院麻醉与围术期医学科,河南开封475001

出　　处：《基础医学与临床》2022年第4期622-627,共6页Basic and Clinical Medicine

基　　金：河南省高等学校重点科研项目计划(19A320021)。

摘　　要：目的探讨氯胺酮对大鼠脊髓缺血/再灌注(SCII)损伤及ERK1/2信号通路的影响。方法将大鼠随机分为假手术组(S组)、SCII组(Zivin法建立模型)、低和高剂量氯胺酮组(分别每天给予50和100 mg/kg氯胺酮,连续14 d);评估大鼠后肢运动情况,检测脊髓组织炎性因子、氧化应激指标;并采用Nissl染色法检测脊髓神经元数量;TUNEL法检测凋亡率;Western blot检测蛋白表达情况。结果与S组相比,SCII组术后Tarlov评分降低,并随时间呈梯度性升高;且脊髓组织正常神经细胞数减少,凋亡率升高;IL-1β、TNF-α、IL-6、VCAM-1水平及丙二醛(MDA)、髓过氧化物酶(MPO)水平均升高(P<0.05),超氧化物歧化酶(SOD)和谷胱甘肽-过氧化物酶(GPX)明显降低(P<0.05),p-ERK1/2表达及p-ERK/ERK1/2比值均升高(P<0.05);与SCII组相比,氯胺酮组上述指标的变化均减轻(P<0.05),且低剂量组更明显。结论低剂量氯胺酮可减轻SCII程度,ERK1/2信号通路可能参与该过程。Objective To investigate the effects of ketamine on spinal cord ischemia-reperfusion injury(SCII)and ERK1/2 signaling pathway in rats.Methods The rats were randomly divided into sham operation group(S group),SCII group(model was established by Zivin method),low and high-dose ketamine groups(given 50 and 100 mg/kg ketamine respectively every day for 14 days),with 8 rats in each group.The hind limb movement of rats was evaluated,inflammatory factors and oxidative stress indexes of spinal cord tissue were detected.The spinal cord neurons were microscopied by Nissl staining,apoptosis rate was detected by TUNEL assay,and protein expression was detected by Western blot.Results Compared with the S group,the Tarlov score in SCII group decreased after surgery and increased gradually with time,the number of normal nerve cells in the spinal cord decreased and the apoptosis rate increased,the levels of IL-1β,TNF-α,IL-6,VCAM-1 and malonaldehyde(MDA),myeloperoxidase(MPO)was found to be increased(P<0.05),superoxide dismutase(SOD)and glutathione peroxidase(GPX)were significantly decreased(P<0.05),the expression of p-ERK1/2 and the ratio of p-ERK/ERK1/2 increased(P<0.05);Compared with SCII group,The changes of above indexes in ketamine group was reduced(P<0.05),and was more obvious in the low dose group.Conclusions Low dose ketamine can reduce the degree of SCII,and ERK1/2 signaling pathway may be involved in this process.

关 键 词：氯胺酮 脊髓缺血/再灌注损伤 信号通路 ERK1/2 

分 类 号：R614[医药卫生—麻醉学]