玉米小斑病抗病QTL的鉴定与效应分析  

Identification of QTL and Effect Analysis of Resistance to Southern Leaf Blight in Maize

作　　者：李志敏[1] 杜文杰 李政 李雪迎 赵萧笛 贾琳[1] LI Zhimin;DU Wenjie;LI Zheng;LI Xueying;ZHAO Xiaodi;JIA Lin(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院,河南郑州450046

出　　处：《信阳农林学院学报》2022年第1期91-94,99,共5页Journal of Xinyang Agriculture and Forestry University

基　　金：河南省教育厅高等学校重点项目(20A210005,20A210019);河南农业大学创新基金项目(30501097)。

摘　　要：为分析玉米小斑病的遗传抗性,分别利用抗病自交系CIMBL29和感病自交系GEMS41为供体亲本和轮回亲本,通过2次回交和3次自交构建了含有179个家系的BC2F4群体。在2017年(河南长葛)和2018年(河南西平)两个环境条件下对群体小斑病抗性进行了评估,结合SNP分子标记构建的高密度遗传连锁图谱,对抗小斑病QTL进行鉴定。QTL定位结果表明,在2个环境中共鉴定了4个抗小斑病QTL,分布在第1、5、6和8染色体上。其中位于第1染色体和第6染色体上的QTL(qSLB1和qSLB6)在2个环境条件下均可以检测到,是稳定的抗病QTL位点,其中,在2017和2018年,qSLB1分别可以解释9.3%和10.9%的抗性表型变异;qSLB6分别可以解释6.3%和6.6%的表型变异。环境稳定型抗病QTL的鉴定为抗病基因精细定位和分子育种奠定了基础。To map the QTL resistance to SLB,a linkage population(BC2F4)containing 179 family lines were developed using CIMBL29 as the resistant donor parent and GEMS41 as the susceptible recurrent parent.The family lines as well as the parental lines were evaluated for resistance to SLB in 2017(Changge)and 2018(Xiping).Combined with the high-density genetic linkage map constructed with SNP markers,the QTL resistance to SLB were detected.The results showed that four QTL were identified in two environments,which were located on chromosomes 1,5,6 and 8.The QTL on chromosomes 1 and 6(i.e.,qSLB1 and qSLB6)are stable QTL across environments,in which qSLB1 can explain 9.3%and 10.9%of the phenotypic variation in 2017 and 2018,respectively,while qSLB6 can explain 6.3%and 6.6%of the phenotypic variation in 2017 and 2018,respectively.The two QTL could be viewed as stable QTL resistance to SLB in maize,which lay the foundation for molecular-assisted selection of the resistance to southern leaf blight in breeding program.

关 键 词：玉米 小斑病 SNP QTL 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]