119份春小麦种质萌发期抗旱性鉴定及抗旱相关基因表达特性分析  

Drought Resistance Identification and Drought-Resistance Related Gene Expression Analysis of 119 Spring Wheat Germplasms at Germination Stage

作　　者：冯举伶 姚立蓉 汪军成[1,2] 司二静[1,2] 杨轲[1,2] 李葆春[1,3] 孟亚雄[1,2] 马小乐[1,2] 尚勋武[2] 王化俊[1,2] FENG Juling;YAO Lirong;WANG Juncheng;SI Erjing;YANG Ke;LI Baochun;MENG Yaxiong;MA Xiaole;SHANG Xunwu;WANG Huajun(Stae Key Laboratory of Aridl and Crop Science Gansu Agricultural University/Gansu Provincial Key Lab of Crop Improvement&Germplasm Enhancement,Lanzhou,Gansu 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730070,China;College of Life Sciences and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China)

机构地区：[1]省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《麦类作物学报》2022年第3期253-263,共11页Journal of Triticeae Crops

基　　金：国家现代农业产业技术体系项目(CARS-05-04B-2);甘肃省教育厅产业支撑计划项目(2021CYZC-12);国家自然科学基金项目(31860377);甘肃省科技支撑计划项目(1604NKC052-2);甘肃省科技重大专项(17ZD2NA016)。

摘　　要：为了解不同小麦种质萌发期抗旱性强弱,以20%PEG6000溶液模拟干旱胁迫,对119份春小麦萌发期种子发芽势、种子发芽率、根鲜重、根干重、苗鲜重、苗干重、叶鲜重、叶干重、根长和苗长进行测定分析,并综合评价其抗旱性。结果表明,种子发芽势、种子发芽率、苗鲜重、苗干重、叶鲜重和苗长可以作为评价春小麦萌发期抗旱能力的主要指标,并筛选出了1份抗旱型材料、36份较抗旱型材料、43份较敏感型材料和39份敏感型材料。为进一步研究抗旱相关基因在抗旱型与干旱敏感型材料萌发期响应干旱胁迫的表达特性,从而验证抗旱材料鉴定的准确性,以筛选出的2份抗旱性最好的种质(蒙茬1号、黑穗)和2份干旱敏感型种质(09594、K250)为材料,利用qRT-PCR对其在不同时长(0h、1h、6h、12h、24h)的20%PEG6000胁迫下TaNAC29、TaC2DP1、TaDLEA3、TaCP、Taα-AMY和TaGPX的表达特性进行分析表明,随着胁迫时间的增加,以上6种基因在小麦叶片和根系中均上调表达,表达量均在胁迫24h时达到峰值,且TaNAC29、TaDLEA3、TaCP和TaGPX在根系中的表达量均明显高于叶片,而TaC2DP1和Taα-AMY在叶片中的表达量更高;此外,抗旱材料蒙茬1号和黑穗中6种基因的表达量均高于敏感材料09594和K250。In order to explore the drought resistance of different wheat germplasms at germination stage,we simulated drought stress with 20% PEG6000 solution,and comprehensively evaluated the drought resistance of 119 spring wheat materials at germination stage by measuring and analyzing the germination potential,germination rate,root fresh weight,root dry weight,seedling fresh weight,seedling dry weight,leaf fresh weight,leaf dry weight,root length and seedling length.The results showed that germination potential,germination rate,seedling dry weight,leaf fresh weight and seedling length could be used as the main indices to evaluate the drought-resistant ability of spring wheat at germination stage.And one drought-resistant material,36 moderate drought-resistant materials,43 moderate sensitive materials and 39 sensitive materials were screened out.In order to further study the expression characteristics of drought-resistant related genes in responding to drought stress at germination stage of drought-resistant and drought-sensitive materials,and simultaneously verify the accuracy of the identification of drought-resistant materials,we used two drought-resistant germplasms(Mengcha 1 and Heisui) and two drought-sensitive germplasms(09594 and K250) as materials,to analyze gene expression characteristics of TaNAC29,TaC2DP1,TaDLEA3, TaCP , Taα-AMY and TaGPX under different time period(0 h,1 h,6 h,12 h,24 h) under 20% PEG6000 stress via qRT-PCR.The results showed that,with the increase of stress time,the above six genes were up-regulated in leaves and roots of wheat,and their expression levels reached the peak at 24 h under drought stress.The expression levels of TaNAC29,TaDLEA3, TaCP and TaGPX in roots were significantly higher than those in leaves,while the expression levels of TaC2DP1 and Taα-AMY were higher in leaves.In addition,the expression levels of the six genes in drought-resistant materials Mengcha 1 and Heisui were higher than those in sensitive materials 09594 and K250.

关 键 词：春小麦 萌发期 抗旱性 QRT-PCR 基因表达量 

分 类 号：S512.1[农业科学—作物学] S330