6种水稻初级代谢物对水稻抗瘟性的影响  

Effects of six metabolites on rice resistance to blast fungus,Magnaporthe oryzae

作　　者：陈红凤 马笑晴 杨荣涛 唐萍[1] 李淑旎 邵世豪 杨静[1] CHEN Hong-feng;MA Xiao-qing;YANG Rong-tao;TANG Ping;LI Shu-ni;SHAO Shi-hao;YANG Jing(State Key Laboratory for Conservation and utilization of Bio-resources in Yunnan/Key Laboratory of Agro-biodiversity and Pest Management of Ministry of Education/College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南生物资源保护与利用国家重点实验室/农业生物多样性与病虫害控制教育部重点实验室/云南农业大学植物保护学院,昆明650201

出　　处：《南方农业学报》2022年第1期57-67,共11页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31860483);云南省基础研究计划重点项目(202101AS070038)。

摘　　要：【目的】解析稻瘟菌与水稻互作时的水稻初级代谢物对稻瘟菌形态发育和水稻防御响应的影响,为生产上水稻稻瘟病绿色防控措施的制定提供基础数据。【方法】利用6种水稻初级代谢物(多巴胺盐酸盐、L-谷氨酰胺、酪胺、α-品烯、L-色氨酸和半乳糖醇)标准品按一定浓度处理稻瘟病菌株,观察代谢物对稻瘟菌菌落生长和菌株形态发育(产孢、菌丝生长和附着胞形成等)的影响;在此基础上,利用相同浓度的代谢物标准品处理稻瘟菌侵染36和48 h时的感病水稻丽江新团黑谷,进行稻瘟病病害调查、防御基因表达分析、活性氧(ROS)含量和过氧化物酶(POD)活性等生理指标测定。【结果】除L-谷氨酰胺和L-色氨酸对生长第9 d的稻瘟病菌菌落生长有显著影响外(P<0.05,下同),其余4种代谢物对稻瘟病菌菌落生长无显著影响(P>0.05,下同);6种水稻初级代谢物对稻瘟菌产孢有抑制作用,其中以酪胺和α-品烯的抑制效果最明显;多巴胺盐酸盐显著抑制稻瘟菌孢子的萌发,其余5种代谢物对稻瘟菌孢子萌发无显著影响;6种水稻初级代谢物对稻瘟菌芽管生长有显著的促进作用;多巴胺盐酸盐和L-色氨酸对附着胞形成有明显的促进作用,L-谷氨酰胺和酪胺对附着胞形成具有抑制作用。6种水稻初级代谢物明显降低稻瘟菌接种36和48 h时的水稻稻瘟病症状和病情指数,其中以多巴胺盐酸盐、酪胺和半乳糖醇对稻瘟病的控制效果最佳;6种水稻初级代谢物对稻瘟菌接种48 h时的稻瘟病控制效果高于其对接菌36 h时的水稻。进一步研究发现多巴胺盐酸盐和半乳糖醇抑制了稻瘟菌侵染36和48 h水稻中茉莉酸(JA)信号基因OsCOI1a、OsCOI1b和OsMYC2表达,但JA抑制子基因OsJAZ9上调,诱导ROS相关基因OsRbohB和OsRbohA以及病程相关基因OsPR10a和OsPR5上调,其中以2种代谢物诱导稻瘟菌侵染48 h时水稻中的ROS相关基因和病程相关基因的表【Objective】Analyzing the effect of primary metabolites during interaction of rice and Magnaporthe oryzae on rice blast morphogenesis and defense response,to provide basic data for the formulation of green control measures for rice blast in production.【Method】The six primary metabolites with a certain concentration(dopamine hydrochloride,L-glutamine,tyramine,α-pinene,L-tryptophan,galactinol)were used to treat the rice blast strains to observe the effects of metabolites on colony growth,strain morphology(sporulation,hypha germination and appressorium formation)of M.oryzae.On this basis,the same concentrations of metabolite standards were used to treat 36 h-and 48 h-infected rice to investigate symptom of rice blast disease,analyze defense gene expression,and detect reactive oxygen species(ROS)content and peroxidase(POD)activity.【Result】Except for L-glutamine and L-tryptophan,which had a significant effect on the growth of the rice blast fungus colony on the ninth day of growth(P<0.05,the same below),the other four metabolites had no significant effect on the growth of the rice blast fungus colony(P>0.05).Six kinds of primary metabolites of rice had inhibitory effect on sporulation of M.oryzae,among which tyramine andα-pinene had the most obvious inhibitory effect.Dopamine hydrochloride significantly inhibited the spore germination of M.oryzae,while the other five metabolites had no obvious effect on the spore germination of M.oryzae.Six kinds of primary metabolites of rice could significantly promote the growth of germ tube of M.oryzae.Dopamine hydrochloride and L-tryptophan promoted appressorium formation,while L-glutamine and tyramine inhibited appressorium formation.Six kinds of primary metabolites in rice reduced the symptoms and disease index of rice blast at 36 and 48 h after inoculation with M.oryzae,among which dopamine hydrochloride,tyramine and galactitol had the best control effect on rice blast.The control effect of the six kinds of primary metabolites on rice blast at 48 h was higher than

关 键 词：水稻 稻瘟病 代谢物 防御相关基因 绿色防控 

分 类 号：S511[农业科学—作物学]