多光子技术可视化血管型脑膜瘤  

Multiphoton Technique for Visualization of Angiomatous Meningiomas

作　　者：方娜 吴赞艺[2] 王行富[3] 林元相[2] 陈建新[4] Fang Na;Wu Zanyi;Wang Xingfu;Lin Yuanxiang;Chen Jianxin(School of Medical Technology and Engineering,Fujian Medical University,Fuzhou,Fujian 350004,China;Department of Neurosurgery,the First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350004,China;Department of Pathology,the First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350004,China;Key Laboratory of OptoElectronic Science and Technology for Medicine,Ministry of Education,Fujian Normal University,Fuzhou,Fujian 350007,China)

机构地区：[1]福建医科大学医学技术与工程学院,福建福州350004 [2]福建医科大学附属第一医院神经外科,福建福州350004 [3]福建医科大学附属第一医院病理科,福建福州350004 [4]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《激光与光电子学进展》2022年第6期320-327,共8页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(62105067);福建省自然科学基金(2021J01133034)。

摘　　要：血管型脑膜瘤血管异常丰富。术前或者术中能明确诊断血管型脑膜瘤对手术方案的制定和预后判断至关重要。联合二次谐波技术、双光子激发荧光技术和光谱技术无标记地对血管型脑膜瘤进行研究。结果表明,多光子技术不仅可以识别血管型脑膜瘤的内源成分,还能可视化它的微结构,联合图像分析技术,还可以进一步自动定位血管的位置并计算血管的数量。随着光纤技术和多光子内窥镜技术的发展,多光子技术有望成为临床诊断血管型脑膜瘤的新技术。Angiomatous meningiomas comprise numerous blood vessels.The ability to clearly diagnose angiomatous meningiomas preoperatively or intraoperatively is crucial for developing surgical plans and prognosis.In this study,we combined the second harmonic,two-photon excited fluorescence,and spectroscopic techniques to realize a label-free study of angiomatous meningiomas.Results show that this multiphoton technique can identify the endogenous components of angiomatous meningioma and visualize its microstructure.Furthermore,combined with image analysis techniques,the multiphoton technique can further automatically locate vessels and calculate number of vessels.With the rapid development in fiber optic and multiphoton endoscopic technologies,multiphoton technique poses as a new technique for the clinical diagnosis of angiomatous meningiomas.

关 键 词：生物技术 多光子技术 双光子激发荧光 二次谐波 血管型脑膜瘤 

分 类 号：O436[机械工程—光学工程]