氟咯草酮影响小鼠血清脂质特征  

The effect of Flurochloridone exposure on serum lipid profile in mice

作　　者：王彦娜 李睿 张芬[1] 周谡 冯超[3] 薛黎明[3] 徐加乐 邬春华[1] 卢大胜[1] 周志俊[1] WANG Yan-na;LI Rui;ZHANG Fen;ZHOU Su;FENG Chao;XUE Li-ming;XU Jia-le;WU Chun-hua;LU Da-sheng;ZHOU Zhi-jun(School of Public Health,Fudan University,Shanghai 200032,China;Shanghai Institute for Drug and Food Control,Shanghai 201203,China;Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]上海市食品药品检验研究院,上海201203 [3]上海市疾病预防控制中心,上海200336

出　　处：《毒理学杂志》2022年第1期15-20,32,共7页Journal of Toxicology

基　　金：国家自然科学基金(81872643)。

摘　　要：目的探究氟咯草酮(Flurochloridone,FLC)暴露对C57BL/6小鼠血清脂质代谢的影响,进一步了解FLC的毒性作用及不良影响。方法采用基于高效液相色谱串联高分辨质谱(HPLC-HRMS)的脂质组学技术分析C57BL/6小鼠染毒FLC后血清脂质代谢的变化。结果375 mg/kg·bw FLC会引起小鼠血清脂质轮廓的改变。对每种脂质种类进行总相对丰度分析发现,FLC可引起溶血性磷脂和神经鞘磷脂明显变化,差异有统计学意义(P<0.05)。差异代谢物筛选发现FLC引起的脂质代谢紊乱涉及甘油磷脂、鞘磷脂和甘油酯等多个脂质代谢过程。结论FLC具有脂毒性,染毒后会引起C57BL/6小鼠脂质代谢紊乱。Objective To explore the effect of flurochloridone(FLC)exposure on serum lipid metabolism in C57 BL/6 mice,and further promotes the understanding of the toxic effects and adverse effects of flurochloridone.Methods The lipidomic techniques based on high performance liquid chromatography tandem high resolution mass spectrometry(HPLC-HRMS)were used to analyze the changes of serum lipid metabolism in C57 BL/6 mice after FLC exposure.Results Flurochloridone at 375 mg/kg·bw induced alterations in serum lipid profiles in mice.Analysis of the total relative abundance of each lipid class found that flurochloridone caused significant changes in lysophospholipids and sphingomyelins(P<0.05).Differential metabolite screening revealed that flurochloridone induced disruption of lipid metabolism involving multiple lipid metabolism processes including glycerophospholipids,sphingolipids and glycerolipids.Conclusion Flurochloridone is lipotoxic and causes lipid metabolism disorders in C57 BL/6 mice.

关 键 词：氟咯草酮 脂质组学 脂质代谢紊乱 溶血性磷脂 神经鞘磷脂 

分 类 号：R99[医药卫生—毒理学] R114[医药卫生—药学]