硒对PM_(2.5)诱导大鼠心肌酶谱变化的影响  

Effect of selenium on myocardial injury induced by PM_(2.5) in rats

作　　者：李晓红[1,2] 欧阳钏 柯鸿阳 单慧 于丽 谭金峰 徐东群 李万伟[1,2] LI Xiao-hong;OUYANG Chuan;KE Hong-yang;SHAN Hui;YU Li;TAN Jin-feng;XU Dong-qun;LI Wan-wei(School of public health,Weifang Medical College,Weifang Shandong 261053,China;Weifang Key Laboratory of health inspection and quarantine,Weifang Shandong 261053,China;School of basic medicine,Weifang Medical College,Weifang Shandong 261053,China;Weifang environmental monitoring center station,Weifang Shandong 261042,China;Institute of environment and health related product safety,China Center for Disease Control and prevention,Beijing 100021,China)

机构地区：[1]潍坊医学院公共卫生学院,山东潍坊261053 [2]潍坊市卫生检验与检疫重点实验室,山东潍坊261053 [3]潍坊医学院基础医学院,山东潍坊261053 [4]潍坊市环境监测中心站,山东潍坊261042 [5]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《毒理学杂志》2022年第1期60-64,共5页Journal of Toxicology

基　　金：山东省医药卫生科技发展计划项目(2019WS592);潍坊市科学技术发展计划(2019GX027);山东省中医药科技项目(2020M077);山东省自然科学基金(ZR 2020MH336)。

摘　　要：目的探讨PM_(2.5)诱导的心肌损伤及硒的保护作用。方法健康清洁级雄性SD大鼠32只,随机分为4组,对照组、PM_(2.5)组、硒组及硒+PM_(2.5)组。分别灌胃给予生理盐水1 ml,或亚硒酸钠溶液35μg/kg·bw后,利用定量雾化器给予1.5 ml生理盐水或140.16μg/ml PM_(2.5)悬液,连续30 d,禁食过夜后,麻醉取血及心脏组织,利用相关试剂盒检测相应指标。结果大鼠暴露PM_(2.5)后,体重增加值较对照组少,硒预处理有所改善;大鼠暴露PM_(2.5),其心肌组织中谷光甘肽过氧化物酶(GSH-Px)及总超氧化物歧化酶(T-SOD)水平较对照组明显降低(P<0.05),丙二醛(MDA)水平较对照组明显增加(P<0.05),硒预处理后,GSH-Px水平较PM_(2.5)组明显升高(P<0.05),MDA水平较PM_(2.5)组明显降低,差异有统计学意义(P<0.05);PM_(2.5)可使大鼠心肌组织中一氧化氮(NO)含量及一氧化氮合酶(NOS)活力水平明显升高(P<0.05),硒预处理可明显降低NO含量及NOS活力水平(P<0.05);PM_(2.5)染毒组大鼠血清中乳酸脱氢酶(LDH)、天冬氨酸转氨酶(AST)、肌酸激酶(CK)及心肌肌钙蛋白T(cTnT)水平明显升高(P<0.05),硒预处理可明显降低这些指标的水平,差异有统计学意义(P<0.05)。结论大鼠暴露一定剂量PM_(2.5)可导致心脏受损,一定剂量硒的干预可在一定程度上起到缓解作用。Objective To investigate the myocardial injury induced by PM_(2.5) and the protective effect of selenium.MethodsA total of 32 SD male rats were randomly divided into four groups:control group,PM_(2.5) group,selenium intervention group and selenium+PM_(2.5) group.1 ml normal saline or 35μg/kg·bw sodium selenite solution was given to rats by gavage,then 1.5 ml normal saline or 140.16μg/kg·bw PM_(2.5) suspension was given via tracheal instillation by using of lung quantitative nebulizer for 30 days.After fasting overnight,blood and cardiac tissue were collected under anesthesia.Some related indexes were detected by relevant kits.Results The body weight gain of rats exposed to PM_(2.5) was less than that of control group,and selenium intervention improved this condition;The levels of GSH-Px and T-SOD in myocardial tissue of PM_(2.5) group were significantly lower than those in control group(P<0.05),and MDA level was significantly increased compared with the control(P<0.05).After selenium intervention,level of GSH-Px was significantly higher than that of PM_(2.5)group(P<0.05),and MDA level was significantly lower than that of PM_(2.5)group(P<0.05);PM_(2.5)could significantly increase the content of NO and the activity of NOS in myocardial tissue(P<0.05),while selenium intervention could significantly reduce the level of NO and NOS activity(P<0.05);The levels of LDH,AST,CK and cTnT in serum of PM_(2.5)group were significantly increased(P<0.05),selenium intervention could significantly dcrease the levels of LDH,CK and cTnT(P<0.05).Conclusion Exposure to a certain dose of PM_(2.5)can cause heart damage,and the intervention of certain dose of selenium can alleviate the damage to a certain extent.

关 键 词：PM_(2.5) 抗氧化酶 心肌酶 硒 

分 类 号：R114[医药卫生—卫生毒理学] R99[医药卫生—公共卫生与预防医学]