动态散射光测量在全场光学相干层析技术中的应用  

Review on Dynamic Scattered Light Measurement in Full-Field Optical Coherence Tomography

作　　者：田浩颍 汤丰锐 高万荣[1] 朱越[1] Tian Haoying;Tang Fengrui;Gao Wanrong;Zhu Yue(School of Electronic and Optical Engineering,Nanjing University of Science and Technology,Nanjing,Jiangsu 210094,China)

机构地区：[1]南京理工大学电子工程与光电技术学院,江苏南京210094

出　　处：《中国激光》2022年第5期108-121,共14页Chinese Journal of Lasers

基　　金：国家自然科学基金青年基金(62005123);江苏省自然科学基金青年基金(BK20190455);教育部中央高校基本科研业务费(30919011226);南京理工大学科研启动经费(AE89991/076)。

摘　　要：全场光学相干层析术(FF-OCT)探测生物组织亚细胞水平折射率扰动的本征信号,为无标记成像提供了丰富的显微结构信息。动态全场光学相干层析技术(D-FFOCT)结合全场OCT低相干层析成像与动态散射光测量原理,可为细胞或组织提供亚细胞动态对比度。介绍了近年来D-FFOCT技术的发展,讨论了基于D-FFOCT的多模态成像技术,分析了成像系统的空间分辨率、信噪比和成像深度。针对目前D-FFOCT干涉信号易受扰动而产生图像伪影缺陷的问题,分析比较了运用高速相机、瞬时移相法、基于希尔伯特变换的算法和基于奇异值分解的图像滤波算法等消除伪影的方法,概述了D-FFOCT在基础生物学、精准医疗领域和药物研究方面的研究,并展望该技术在这些领域的发展前景和未来研究方向。Significance Optical coherence tomography(OCT)is a label-free,non-invasive,and high-resolution coherent imaging technology coupled in many fields,such as biology,medicine,and materials.It can obtain cross-sections in subcellular resolution and accumulate microstructures in three-dimensional space with high-isotropic spatial resolution.Arising from the early 90s,during its development,higher resolution,more contrasts on specific structures,and wider range of applications are its main development directions.Based on time-domain OCT,the full-field OCT(FF-OCT)employs an identical pair of high numerical aperture microscopic objectives and a low-cost and broadband white light source to improve the imaging resolution of the system to the submicron level.It is known that different structures of biological tissues,such as nuclei and cell media,have different backscattering capabilities of light.FF-OCT detects and extracts intrinsic signals related to refractive index variance of biological tissues at the subcellular level.However,the performance of FF-OCT can be tricky when the interference of strongly scattering media reduces image contrast during single-cell imaging,and it will lose behavioral information during metabolism activities.Recently,a dynamic FF-OCT(D-FFOCT)shows the ability to visualize metabolic contrast in mouse organoid,which bring a new imaging mechanism to deal with those problems.The idea combines the principles of FF-OCT and dynamic scattered light measurement.It can detect subcellular dynamic activities for cells or tissues.The special distinguishing ability of D-FFOCT makes it possible to differentiate the types and functions of cells.Since D-FFOCT was first proposed,some scientific studies have been reported.Therefore,it is necessary to summarize the existing research to extract the principle key points and attract more attention for related researchers.Progress First,the current D-FFOCT technology development is described,and the tradeoff between functions and performance is elaborated.D-FFOCT was

关 键 词：生物光学 无标记成像 全场光学相干断层扫描技术 动态散射光测量 图像伪影 精准医疗 

分 类 号：O436[机械工程—光学工程]