基于空间频域成像的人体皮肤组织光学和生理参数的提取  

An Approach for Extracting Optical and Physiological Parameters of Human Skin Tissue Based on Spatial Frequency Domain Imaging

作　　者：康旭 张洋 任慧明 张元志 王贻坤 Kang Xu;Zhang Yang;Ren Huiming;Zhang Yuanzhi;Wang Yikun(Institutes of Physical Science and Information Technology,Anhui University,Hefei,Anhui 230601,China;Anhui Proviyicial Engineering Laboratory for Medical Optical Diagnosis&Treatment Technology and Instrument,Anhui Insititute of Optics and Fine Mechanics,Hefei Institute of Physical Science,Chinese Academy of Sciences,Hefei,Anhui 230026,China)

机构地区：[1]安徽大学物质科学与信息技术研究院,安徽合肥230601 [2]中国科学院合肥物质科学研究院安徽光学精密机械研究所,安徽省医用光学诊疗技术与装备工程实验室,安徽合肥230026

出　　处：《中国激光》2022年第5期188-197,共10页Chinese Journal of Lasers

基　　金：安徽省重点研究与开发计划(202004a07020016);安徽省自然科学基金(1908085QH365);中国科学院合肥物质科学研究院院长基金(YZJJZX202009);中国科学院科技服务网络计划(KFJ-STS-QYZD-184)。

摘　　要：人体皮肤组织的光学参数和生理参数对于众多疾病的检测与监测具有重要意义,基于空间频域成像技术提取光学、生理参数具有潜在的临床应用价值。空间频域成像技术需要至少在两个波长下提取氧合血红蛋白和脱氧血红蛋白浓度;在反演人体皮肤组织中的氧合血红蛋白和脱氧血红蛋白浓度时,皮肤中的黑色素会增加结果的不确定度。本课题组搭建了一套空间频域成像系统,在反演人体皮肤组织生理参数的算法中引入最小二乘法和最小误差准则,以解决不确定度问题。实验结果表明:该系统获得的梯度漫反射板的测量值与准确值线性相关,且获取的组织仿体的吸收系数与直接测量法获取的吸收系数的偏差在0.3%~9.6%之间,验证了该系统测量光学参数的有效性。通过对不同黑色素含量的组织仿体进行测量后发现,系统的测量浓度与配制浓度之间显著相关。血液仿体实验和动脉闭塞实验结果表明,系统测量的氧合血红蛋白、脱氧血红蛋白、总血红蛋白浓度以及血氧饱和度随时间变化的趋势符合血液实验变化趋势和闭塞条件的变化趋势,进一步验证了空间频域成像算法提取生理参数的准确性。在体和离体实验结果均说明空间频域成像方法可以用于组织体中血氧饱和度的测量,为实现相关疾病的无创、快速检测提供了新手段。Objective The optical and physiological parameters of human skin tissue are of extraordinary value to detect and monitor many diseases.The optical and physiological parameter extraction based on spatial frequency domain imaging(SFDI)technology has practicable clinical utility value.Microvascular oxygen saturation carries the statistics of oxygen transport and consumption rate of skin and subcutaneous tissue.Monitoring of microvascular oxygen saturation has the necessary magnitude in clinical diagnosis of microcirculation and metabolism and basic research of chronic diseases.The traditional blood oxygen saturation measurement measures blood oxygen partial pressure by percutaneous oxygen partial pressure analyzer and then calculates blood oxygen saturation.However,this approach has low sensitivity,long measurement time and can only be measured by single point contact.The spatial frequency imaging technique can be used with a medium resolution to quickly measure large areas with splendid clinical value.Methods SFDI requires the extraction of oxygenated and deoxygenated hemoglobin at least two wavelengths.Skin melanin increases the uncertainty of results when retrieving oxygenated and deoxygenated hemoglobin of human skin tissue.In this study,a set of SFDI system was developed.Additionally,the least square method and minimum error criterion were introduced into the algorithm of retrieving physiological parameters of human skin tissue to solve the uncertainty problem.LED chips with central wavelengths of 529 and 617 nm were used in the lighting module of the SFDI system.After coupling and collimation,the illumination light was irradiated onto the digital micromirror device(DMD)and spatially modulated to form a wide field sinusoidal pattern.Then,it was projected onto the skin tissue to be measured.A complementary metal oxide semiconductor(CMOS)camera was used to collect the diffuse reflectance of the tissue.The three-phase displacement method was used to obtain the diffuse reflectance of the skin tissue.The optical par

关 键 词：医学光学 空间频域成像 最小误差准则 参数提取 

分 类 号：TN29[电子电信—物理电子学]