Hypocotyl elongation based on HY5 transcription factor in cold resistant winter rapeseed(Brassica napus L.)  

作　　者：Jiaojiao Jin Wancang Sun Junyan Wu Yan Fang Xuecai Li Li Ma Lijun Liu Rui Zeng 

机构地区：[1]College of Agriculture and Forestry,Longdong University/Gansu Province Dryland Winter Wheat Germplasm Innovation and Application Engineering Research Center/Provincial Collaborative Innovation Center,Qingyang,745000,China [2]College of Agronomy,Gansu Agricultural University/Gansu Provincial Key Laboratory of Arid Land Crop Sciences/Gansu Research Center of Rapeseed Engineering and Technology/Key Laboratory of Crop Genetics Improvement and Germplasm Enhancement of Gansu Province,Lanzhou,730070,China

出　　处：《Oil Crop Science》2022年第1期40-52,共13页中国油料作物学报(英文版）

基　　金：Longdong College youth Science and technology innovation project(XYZK2106);the Agriculture Research System Gansu Province(GARS-TSZ-1);Agriculture Research System of China(CARS-12);Study on variety substitution and key technology of cold resistance and high yield of winter rape(GSLK-2021-12);the National Natural Science Foundation of China(31860388)。

摘　　要：Winter rape(Brassica napus L.)is better than other edible oil crops in China,but poor cold resistance is the key factor restricting its development.Hypocotyl length was found closely related to cold tolerance.The correlation between hypocotyl length and semi-lethal low temperature was significant,and the highest correlation between hypocotyl length and LT50 of autumn sowing was 0.9557.When the hypocotyl were treated at low temperature,the cells were seriously damaged and formed cavity structures,with cell walls seriously damaged or merged into each other.The positive regulation gene of hypocotyl length in resistant line of VHTSG 10 was identified as HY5(transcription factor HY5-like)by qPCR,and bZIP transcription factor was found to be its conserved domain.Fused gene by GFP and HY5 from VHTSG 10 was transient transferred into Nicotiana benthamiana cells.Corresponding to the 35S:GFP widely distributed in plasma membrane of leaf epidermis,the fusion protein 35S:HY5-GFP was mainly distributed in nucleus.Thus we regarded BnHY5 gene is a key gene related to cold tolerance and hypocotyl length in B.napus.

关 键 词：Semi-lethal low temperature(LT50) Morphological physiology HY5 gene Subcellular localization 

分 类 号：S565.4[农业科学—作物学]