越南紫薯组培快繁及FMV病毒检测  

Tissue Rapid Propagation and FMV Virus Detection of Vietnam Ipomoea batatas

作　　者：李艳辉 穆玉洁 白冰洋 蒋素华[3] LI Yan-hui;MU Yu-jie;BAI Bing-yang(Fengqiu County Agriculture and Rural Affairs Bureau,Xinxiang,Henan 453000;Luohe Tiankang Agricultural Development Co.,Ltd.,Luohe,Henan 462000)

机构地区：[1]封丘县农业农村局,河南新乡453000 [2]漯河田康农业发展有限公司,河南漯河462000 [3]郑州师范学院生物工程研究中心,河南郑州450044

出　　处：《安徽农业科学》2022年第7期92-95,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省科技攻关计划项目(202102110167);河南省高等学校重点科研项目(21A210029);河南省高等学校重点科研项目(21B210011)。

摘　　要：[目的]探究获得大规模的越南紫薯脱毒苗的技术方法。[方法]采用茎尖组织培养快繁技术和RT-PCR技术,探讨不同培养基成分对越南紫薯无毒茎尖的诱导、分化、增殖以及生根的影响和羽状斑驳病毒的检测,建立越南紫薯高效再生体系和病毒检测体系。[结果]筛选出最适越南紫薯不定芽产生的培养基是MS+6-BA 1.0 mg/L,最佳增殖培养基为1/2MS+6-BA 1.0 mg/L+CW 2.0 mg/L,最佳生根培养基为1/2MS+NAA 0.5 mg/L。[结论]该试验为大田甘薯种苗FMV病毒的高效检测提供了理论依据。[Objective]To acquire large-scale Vietnam Ipomoea batatas seedings.[Method]This study adopts the tissue of rapid propagation technology,discusses different culture medium components of Vietnam Ipomoea batatas buds reduction,differentiation,proliferation and the influence of root.[Result]The results illustrate that the MS+6-BA 1.0 mg/L was the optimum medium for Ipomoea batatas buds reduction,1/2MS+6-BA 1.0 mg/L+CW 2.0 g/L was the best medium for Ipomoea batatas proliferation,1/2MS+NAA 0.5 mg/L was the optimum medium for Ipomoea batatas growing roots.[Conclusion]Therefore,this experiment can provide a fundamental theory for virus G detection in the field.

关 键 词：越南紫薯 FMV病毒 组织培养 

分 类 号：S531[农业科学—作物学]